穿心莲片中穿心莲内酯的薄层扫描

TLCS OF ANDROGRAPHOLIDE IN ANDROGRAPHITIS TABLETS

对穿心莲内酯的测定方法为:先在硅胶GF_(254)荧光薄层板上用氯仿-无水乙醇(10:1)为展开剂分离穿心莲内酯及其它成分,再用岛津CS-910型双波长薄层扫描仪在紫外区进行荧光熄灭定量扫描(测定波长240nm,参比波长400nm),然后按公式计算含量。其回收率为96.0％(n=5),变异系数为2.1％。

Andrographolide and other components were separated on sillica gel G F_(254) fluorescence plate with chloroform-anhydrous alcohol (10:1) as developing sol- vent. Scanning by a Shimadzu TLCS CS-910 with quenching to determine an- drographolide in andrographitis tablets was performed at 240 nm, (as ref. wa- velength 400 nm). The average recovery of andrographolide in andrographitis tablets was 96,0% (n=5) with variation coefficient of 2.1%.