蜂王浆冻干粉对小鼠脾细胞增殖的影响

Royal jelly producing effects on mice spleen cell proliferation

目的:观察蜂王浆冻干粉(lyophilized royal jelly,LRJ)对小鼠脾细胞增殖的影响。方法:MTT法检测LRJ溶液及含药血清对体外培养小鼠脾细胞和口服LRJ对小鼠脾细胞增殖的影响。结果:LRJ溶液能剂量依耐性的降低静息脾细胞活性,抑制ConA活化的脾细胞的增殖活性。而含药血清对静息和活化的脾细胞的增殖活性均表现出促进作用,和空白血清比较有显著性差异(P<0.01)。口服LRJ的小鼠其脾细胞的增殖活性大于正常对照组,有显著性差异(P<0.01)。结论:LRJ作用于机体后,能刺激机体产生活性物质,提高脾细胞的增殖活性,调节机体免疫力。

Abstact: objective: to observe the royal jelly producing(lyophilized royal jelly, LRJ) affect the spleen cell proliferation in mice. Methods: determined by MTT method to detect LRJ solution and drug-containing serum on in vitro culture of mouse spleen cells and oral LRJ effects on mice spleen cell proliferation. Results: LRJ solution can reduce resting dose dependency of spleen cell activity and inhibition of ConA activated spleen cell proliferation activity. The drug-containing serum of resting and activating the spleen cell proliferation activity of promoting effect, compared with blank serum had significant difference(P < 0.01). Oral LRJ mice the spleen cell proliferation activity is greater than the normal control group, with significant difference(P < 0.01). Conclusion: after LRJ ACTS on the body, can stimulate the body to produce active material, improve the activity of spleen cells proliferation, regulate the body's immune system.