新疆地区维吾尔族与哈萨克族和汉族疼痛敏感性与A118G基因多态性的相关性研究

Relation between pain sensitivity and A118G gene polymorphism in Uygur,Kazak and Han nationalities in Xinjiang region

目的探讨新疆地区维吾尔族、哈萨克族和汉族阑尾手术患者疼痛敏感性与A118G基因多态性的相关性。方法完全随机选取2016年1月至2017年11月在新疆维吾尔自治区人民医院接受阑尾手术麻醉的维吾尔族、哈萨克族、汉族患者各50例。采集患者静脉血,通过聚合酶链反应-限制性片段长度多态性检测技术检测A118G基因多态性;通过压力刺激检测患者试验性疼痛中的压力痛阈和压力耐痛阈;采用疼痛视觉模拟量表(VAS)评分记录患者术后6、12、24、48 h评分并记录术后48 h内不良反应发生情况。结果维吾尔族患者G等位基因频率明显高于哈萨克族和汉族患者[41. 0%(41/100)比26. 0%(26/100)、19. 0%(19/100)](均P <0. 05)。3组患者压力痛阈比较差异无统计学意义(P=0. 621),维吾尔族和哈萨克族患者压力耐痛阈总均值均低于汉族患者(均P <0. 05),3组中GG基因型携带者压力痛阈和压力耐痛阈均低于AA基因型和AG基因型携带者(均P <0. 05)。术后6 h维吾尔族与哈萨克族患者VAS评分总分均高于汉族患者[(8. 13±0. 87)、(8. 32±1. 07)分比(7. 92±0. 62)分](均P <0. 05),维吾尔族与汉族携带GG基因型患者术后6、12、24、48 h的VAS评分均明显高于同族其他基因型患者(均P <0. 05)。3组患者术后48 h内不良反应发生率比较差异无统计学意义(P=0. 816)。结论 A118G基因多态性与新疆地区维吾尔族、哈萨克族和汉族阑尾手术患者疼痛敏感性相关,G等位基因突变可能是增加疼痛敏感性的直接因素。

Objective To investigate the relation between pain sensitivity and A118 G gene polymorphism in Uygur,Kazak and Han patients undergoing appendix operation in Xinjiang region. Methods From January2016 to November 2017,respectively 50 cases of Uygur,Kazak and Han patients undergoing appendectomy in People’s Hospital of Xinjiang Uygur Autonomous Region were enrolled. Venous blood was sampled and A118 G gene polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism. Pressure pain threshold( PPT) and pressure pain tolerance( PTO) were detected by stress stimulation. Pain Visual Analogue Scale( VAS) scoring was assessed 6,12,24,48 h after operation and postoperative adverse reactions were observed within 48 h. Results Frequency of G allele in Uygur was significantly higher than that in Kazak and Han[41. 0%( 41/100) vs 26. 0%( 26/100),19. 0%( 19/100) ]( both P < 0. 05). PPT showed no significant difference among Uygur,Kazak and Han( P = 0. 621). PTO in Uygur and Kazak were significantly lower than that in Han( both P < 0. 05). PTO and PPT in GG genotype carriers were significantly lower than those in AA and AG carriers( all P < 0. 05). At 6 h after operation,VAS scores in Uygur and Kazak were significantly higher than that in Han[( 8. 13 ± 0. 87),( 8. 32 ± 1. 07) vs( 7. 92 ± 0. 62) ]( both P < 0. 05). VAS scores 6,12,24 and 48 h after operation in GG genotype carriers of Uygur and Han were significantly higher than those in other genotypes( all P < 0. 05). There was no significant difference of the incidence of adverse reactions among the 3 groups( P =0. 816). Conclusions A118 G gene polymorphism is associated with pain sensitivity in Uygur,Kazak and Han nationalities in Xinjiang. G allele mutation may be an influence factor of pain sensitivity.