人参土芽孢杆菌用于胰酪大豆胨琼脂培养基的质量控制研究

A Study on the Quality Control of Bacillus Ginsengihumi Used in TSA

目的:通过考察辅料检出的人参土芽孢杆菌(Bacillus ginsengihumi)在不同胰酪大豆胨琼脂培养基(TSA)的生长情况反映培养基的性能差异,讨论菌株作为TSA培养基适用性试验用菌的可行性。方法:将带菌辅料和经多项鉴定的人参土芽孢杆菌分别接种至8个厂家的TSA培养基和TSA对照培养基,讨论9种TSA培养基对辅料污染微生物的检出能力,以及8家企业TSA培养基对人参土芽孢杆菌的促生长能力。结果:由于该辅料携带微生物对营养要求的特殊性,部分厂家TSA检出能力不足,需氧菌总数检查结果偏低。仅B、E两个厂家培养基的促生长能力较高(均大于70%),结果与检出能力一致。结论:质控实验室根据实际需要,可将人参土芽孢杆菌作为TSA质量控制菌株之一。通过本文的讨论模式,各质控实验室也可增加生产环境或产品中的分离菌株,以用于培养基的质量评价,确保培养基对样品检测的灵敏度和可信度,使检验方法更加科学,检验结果更加准确。

Objective: To discuss the feasibility of using Bacillus ginsengihumi in the applicability test of Tryptic Soy Agar(TSA) according to the difference in the performance of TSA which is reflected from observing the growth of Bacillus ginsengihumi isolated from an auxiliary material in different TSAs. Methods: The growth promoting ability and inspection ability of the TSA culture medium and TSA reference medium from eight brands were tested by inoculating the tested excipient and the fresh culture of Bacillus ginsengihumi which was identified by using multiphase identification method. Results: There were significant differences in the growth promoting ability and inspection ability for Bacillus ginsengihumi among the eight manufacturers, and brand B and brand E were the best(>70%). Conclusion: It is concluded that Bacillus ginsengihumi will be used as one of the quality control strains in the TSA quality control system. Based on this paper’s discussion, the quality control laboratories can increase isolation strains in the production environment or products in the quality evaluation of culture medium so as to promote the sensitivity and reliability of the medium and to make the microbiology limit test more scientific and accurate.