摘要
目的:对在保健食品中发现的一种非法添加的西地那非衍生物进行确证。方法:采用高效液相色谱-二极管阵列检测器(HPLC-DAD)技术在日常检测保健食品非法添加时发现一种新型西地那非衍生物。经制备液相法分离纯化得到该化合物,采用高效液相色谱-二级质谱联用(LC-MS/MS)技术获得该化合物准分子离子和碎片离子,并进一步通过核磁共振氢谱确认,结合文献分析,最终确证该化合物。结果:该化合物分子式C_(23)H_(30)N_6O_3,分子量438,结构为去甲基卡波地那非。结论:去甲基卡波地那非在国内未被报道,是一种新型PDE-5抑制剂。
Objective:To confirm one kind of sildenafil derivatives illegally added in health foods.Methods:A novel sildenafil derivative was observed in a routine screening for compounds illegally added in heath foods by HPLC-DAD.The compound was purified by preparative HPLC.Its quasi-molecular ion and MS2 fragmentation spectrum were obtained by UPLC-MS/MS and was further confirmed by 1 H-NMR.The structure of the compound was elucidated by analysis of these data and literature review.Results:The formula of this compound was C23H30N6O3 with molecular weight of 438,and the structure was Desmethyl Carbodenafil.Conclusion:Desmethyl Carbodenafil has not been reported in China yet and is a novel PDE-5 inhibitor.
作者
王珂
董培智
申国华
张禄
梁卜文
郭景文
Wang Ke;Dong Peizhi;Shen Guohua;Zhang Lu;Liang Buwen;Guo Jingwen(Shanxi University of Chinese Medicine,Jinzhong 030619,China;Shanxi Institute for Food and Drug Control,Taiyuan 030001,China)
出处
《中国药事》
CAS
2019年第4期429-434,共6页
Chinese Pharmaceutical Affairs
关键词
保健食品
非法添加
西地那非衍生物
PDE-5抑制剂
去甲基卡波地那非
液质联用
核磁共振
health foods
illegally added
sildenafil derivatives
PDE-5 inhibitor
Desmethyl Carbodenafil
liquid chromatograph-mass spectrometer
nuclear magnetic resonance
