摘要
目的:研究影响特种獭兔毛色的相关基因,讨论其与毛色的相关性。方法:本次研究通过比对NCBI数据库中不同兔种的MC1R,以突变位点为基础设计引物,采用PCR技术对MC1R基因的不同片段进行扩增,然后将扩增产物进行测序,再对MC1R片段进行序列比对从而找出其突变位点,并分析其毛色性状的相关性。结论:通过扩增产物序列之间的对比和产物序列与NCBI数据库碱基序列的对比,得到以下结论:MC1R扩增片段序列的第40位点存在T→C的突变,为獭兔特有突变位点;第50位点存在A→G碱基突变,发生突变序列所对应的毛色性状为白色、海狸色和褐色;第45位点后缺失一段长度为23bp的碱基序列,该缺失可能与兔种有关;第507位点存在T→C的碱基突变,发生突变的序列对应的毛色性状为海狸色。
Objective:The objective of this study was to research on the effect of related gene to special hair col?or of Rex rabbit and discuss the correlation between genes and hair color. Method:Through the comparison of different rabbit’MC1R from NCBI database, design the primer based on mutation site.The different fragments of MC1R gene was amplified by PCR technology, then the amplified products were sequenced. Compare the MC1R fragment sequence and identify mutation sites and analysis the correlation about the hair color.Conclu?sion:Through the comparison the sequences from the amplified products and those with the NCBI database.We concluded that the 40th locus on the sequence of MC1R amplified fragment was mutation from T to C. This is the unique mutation of Rex Rabbit.The 50th locus was mutation from A to G.The hair color corresponding to the occurrence of the mutation sequence is white, beaver color,and brown.The length of 23bp gene was defected af?ter 45th sites.This loss may be related with the rabbit species.The 507th locus was mutation from T to C.The hair color corresponding to the occurrence of the mutation sequence is beaver color.
出处
《中国养兔》
2015年第3期4-9,共6页
Chinese Journal of Rabbit Farming
