尤斯室系统研究大蒜辣素在大鼠不同肠段的吸收特性

Absorption Study of Allicin in Different Intestinal Segments in Rats by Ussing Chamber System

目的研究大蒜辣素在大鼠肠道的吸收特性。方法首先建立高效液相色谱法(HPLC)测定大鼠肠液中大蒜辣素含量测定的分析方法,进行完整的方法学验证;采用尤斯室系统测定不同浓度大蒜辣素溶液在大鼠十二指肠、空肠、回肠、结肠4个肠段的吸收特性。结果建立HPLC测定大鼠肠液中大蒜辣素含量的分析方法;尤斯室测定大鼠各肠段的吸收速率常数在实验浓度范围内随大蒜辣素质量浓度的增加而增加,大蒜辣素在大鼠不同肠段的吸收均为线性吸收,其回归相关系数(r)均达到0. 961 6,符合零级吸收。结论大蒜辣素在大鼠不同肠段的吸收特性不同,同时可以原型形式被吸收。

OBJECTIVE To study the absorption characteristics of allicin in rat intestinal tract.METHODS An HPLC method was established to determine the content of allicin in rat entericus,and Ussing chamber system was used to investigate the absorption characteristics of garlic spicy element of different concentrations in the duodenum,jejunum,ileum and colon of rats.RESULTS An HPLC method was established to determine the content of allicin in rat intestinal liquid.The absorption rate constants of allicin in various intestinal segments of rats in the experimental concentration range increased with the increase of allicin concentration.Allicin showed linear absorption in different intestinal segments of rats,with regression correlation coefficients of greater than 0.96,indicating zero order absorption.CONCLUSION Allicin has different absorption characteristics in different intestinal segments of rats and can be absorbed as prototype.