UPLC/Q-TRAP-MS分析一元羧酸转运体对白头翁五环三萜皂苷吸收的影响

The effect of Monocarboxylic acid (MCTs) on the absorption of pulchinenosides analyzed by UPLC/Q-TRAP-MS

目的:考察白头翁五环三萜皂苷B3, BD, B7, B10, B11在十二指肠、空肠、回肠中的膜通透性,并研究小肠上皮MCTs对5种五环三萜皂苷吸收的影响。方法:采用大鼠外翻肠囊实验,建立超高效液相色谱-三重四级-线性离子肼-质谱(UPLC/Q-TRAP-MS)法,通过一元羧酸转运体(MCTs)抑制剂阿魏酸、苯甲酸和布洛芬实验进行考察。结果:5种白头翁皂苷类成分在十二指肠段中的表观渗透率(P_(app))最高(P<0.05或P<0.01),空肠段次之,回肠段最小;阿魏酸、苯甲酸、布洛芬在十二指肠、空肠、回肠中对白头翁皂苷均有抑制作用(P<0.05,P<0.01)。结论:十二指肠可能为该类皂苷吸收的主要部位;白头翁皂苷可能为MCTs的底物。

OBJECTIVE To investigate the membrane permeability of Pulsatilla pentacyclic triterpenoid saponins(Pulchinenosides)B3,BD,B7,B10,B11 in duodenum,jejunum and ileum and to study the effect of monocarboxylic acid transporters(MCTs)on the absorption of Pulchinenosides in the intestinal epithelial cells.METHODS Ultra-high performance liquid chromatography-triple quadrupole-linear ion trap-mass spectrometry(UPLC/Q-TRAP-MS)was established by everted gut sac test in rats.Tests via the co-administration of MCTs’inhibitors ferulic acid,benzoic acid and ibuprofen were performed.RESULTS The apparent permeability(Papp)of the pulchinenosides was the highest in the duodenum(P<0.05 or P<0.01),followed by the jejunum and the ileum.and all of three MCTs inhibitors ferulic acid,benzoic acid and ibuprofen showed inhibitory effects on the absorption of pulchinenosides in duodenum,jejunum and ileum(P<0.05,P<0.01).CONCLUSION The duodenum was probably the main site for the absorption of pulchinenosides and pulchinenosides was probably the substrates of the MCTs.