督脉不同穴位组合电针对急性上颈段脊髓损伤影响的实验研究

Effects of Different Du Meridian Electro-acupuncture Combinations for Rat with Acute Severe Upper Cervical Spinal Cord Compression Injury

目的:探究督脉不同穴位组合电针刺激对急性重度上颈髓损伤大鼠的治疗效果。方法:SPF级Wistar大鼠(30只)随机分为5组(假手术组、激素组、电针1组、电针2组、模型组,每组6只),分别给予不同的干预方法,干预结束后取材。检测方法:Basso-Beattie-Bresnahan(BBB)的行为学评分,酶联免疫吸附实验法(ELISA)测定脊髓损伤组织血小板活化因子(PAF)含量,免疫印迹试验(Western Blot)法测定脊髓损伤组织Caspase-9表达。结果:BBB评分:假手术组在各个时间点均高于其他组,激素组与电针1组评分相近,但高于电针2组和模型组,电针2组高于模型组。PAF含量测定结果:假手术组的PAF含量低于其他组,电针1组低于激素组、电针2组和模型组,激素组低于电针2组及模型组,电针2组低于模型组。Caspase-9表达结果:假手术组的PAF含量低于其他组,电针1组低于激素组、电针2组和模型组,激素组低于电针2组及模型组,电针2组低于模型组。结论:在脊髓减压的基础上,能够使电流通过损伤组织的督脉穴位组合电针治疗急性上颈髓损伤较未能使电流通过损伤组织的督脉穴位组合电针和单纯脊髓减压效果更佳,其作用机制可能与抑制细胞凋亡和改善脊髓损伤部位微环境有关。

Objective:To verify the treatment effect that different Du meridian electro-acupuncture combinations for rat with acute severe upper cervical spinal cord compression injury.Methods:All the rats were divided into five groups randomly:sham,methylprednisolone(MP),EA-1,EA-2,DE,6 each group.Giving them different interventionsand obtaining experimental materials after interventions.Methods used:BBB score to evaluate the change of each group hind limbs motor function.The ELISA method to assess the change of the content of platelet activating factor(PAF)in injured spinal cord tissue.Western Blot method to assess the Caspase-9 expression for each group.Results:BBB score:score of the sham groups were higher than the other groups,score of the MP group and EA-1 group was similar and higher than the EA-2 and DE group,score of the EA-2 group was higher than the DE group.The content of PAF,the PAF contents of all the groups were sorted from low to high,that is,the sham group,the EA-1 group,the MP group,the EA-2 group,the DE group.The caspase-9 expression:the caspase-9 expression of all the groups were sorted from low to high,that is,the sham group,the EA-1 group,the MP group,the EA-2 group,the DE group.Conclusion:Governor vessel electro-acupuncture which got the electricity through the damaged tissue on acute severe upper cervical spinal cord compression injury had a better effect than that which did not get the electricity through the damaged tissue and decompression only.