通络汤对骨性关节炎模型髓样分化因子及相关蛋白的影响研究

Effect of Tongluo Decoction on Myeloid Differentiation Factor and Related Proteins in Osteoarthritis Model

目的:探究通络汤对大鼠膝关节骨性关节炎模型TOLL样受体(TLR4)/髓样分化因子88(MYD88)信号通路相关蛋白表达的影响。方法:将40只SD成年大鼠,取出10只作为正常组,其余30只建立KOA模型成功后,随机分为模型组、氨糖组和通络组,每组10只。模型组给予纯水,氨糖组给予盐酸氨基葡萄糖胶囊,通络组给予中药通络汤,连续灌喂2个月。2个月后对实验动物进行形态学和组织学观察并测定TLR4/MYD88蛋白的表达。结果:模型组较氨糖组与通络组TLR4/MYD88蛋白阳性表达显著增高,差异有统计学意义(P<0.05);通络组与氨糖组相比,通络组TLR4/MYD88蛋白表达更低,差异有统计学意义(P<0.05)。结论:通络汤能有效减缓大鼠KOA模型软骨退变,抑制滑膜炎症。

Objective:To investigate the effect of Tongluo decoction on the expression of TOLL-like receptor(TLR4)/myeloid differentiation factor 88(MYD88)signaling pathway-related protein in rat knee osteoarthritis model.Methods:40 SD adult rats were taken out as normal group,and the remaining 30 were established with KOA model.After successful,they were randomly divided into model group,amino sugar group and Tongluo group,with 10 rats in each group.The model group was given pure water,the amino sugar group was given glucosamine hydrochloride capsule,and the Tongluo group was given traditional Chinese medicine Tongluo decoction,which was continuously fed for 2 months.After 2 months,the experimental animals were subjected to morphological and histological observation and the expression of TLR4/MYD88 protein was determined.Results:The positive expression of TLR4/MYD88 protein in the model group was significantly higher than that in the glycosaminoglycan group and the Tongluo group(P<0.05).The expression of TLR4/MYD88 protein in the Tongluo group was lower than that in the glycosyl group(P<0.05).Conclusion:Tongluo decoction can effectively alleviate cartilage degeneration in rat KOA model and inhibit synovial inflammation.