用于骨质疏松脊柱压缩骨折的新型可降解材料的体外实验研究

Development of a new degradable bioglass-based bone cement for osteoporotic compressive fractures:an in vitro experimental study

目的研发一种用于治疗骨质疏松椎体压缩骨折的新型复合生物玻璃的磷酸钙骨水泥,并观察其体外材料学及生物学活性。方法将不同质量百分比的生物玻璃(bioglass,BG)与磷酸钙(calcium phosphate cement,CPC)球磨后物理共混获得一种具有可注射、自固化、可降解的椎体成型替代材料。分别对该替代材料的凝固时间、流动性、力学强度进行测定并行材料体外成骨细胞粘附实验、增殖实验,观察其生物相容性。结果随着新型替代材料中BG组分含量的增加,替代材料的凝固时间逐渐延长,同时,流动性较磷酸钙骨水泥改善明显。随着凝固时间的延长,替代材料固化后的抗压强度显著高于实验组CPC骨水泥。此外,与CPC相比,替代材料更有利于细胞的粘附、增殖及分化,具有良好的生物相容性。结论新型复合生物玻璃的磷酸钙的生物材料不仅具有可注射性和较高的力学强度,同时骨传导性能更好,有希望成为临床治疗骨质疏松椎体压缩骨折的一种新型椎体成形材料。

Objective To develop a newtype of bone cements by adding bioglass( BG) into calcium phosphate cement( CPC)and evaluate its material properties and bioactivity. Methods Different proportions of the CPC-BG composite were mixed to obtain an injectable,self-solidified,and degradable alternative material. The osteoblasts( OB) were seeded onto the CPC-BG composite disks and the adhesion,proliferation,morphology,and differentiation abilities of the OB were observed. Results The setting times of CPC-BG were prolonged as the content of BG increased. The injectability of CPC-BG composite paste improved significantly compared to that of CPC paste( P < 0. 05). The compressive strength of CPC-BG composites was higher than that of CPC significantly( P < 0. 05). In addition,the degree of cell attachment,proliferation and differentiation,and biocompatibility increased by adding BG into CPC. Conclusion CPC-BG with injectability and high strength and good bioactivity,may become a newmaterial for vertebroplasy in the treatment of osteoporotic compressive fractures.