TNF-α、iNOS在中药补肾固筋方治疗羊膝骨性关节炎中的表达及修复意义

Expression and significance of tumor necrosis factor-alpha and inducible nitric oxide synthase in knee osteoarthritis treated with TCM tonifying kidney and consolidating tendon prescription in goats

目的研究补肾固筋方对骨关节炎(knee osteoarthritis,KOA)TNF-α、i NOS的作用调节机制。方法 0.3岁雄性山羊72只,随机分为正常组、模型组、西药组、中药组,每组18只,改良Hulth造模,取血清及股骨内髁关节软骨滑膜及软骨下骨,ELISA法检测TNF-α、i NOS。结果西药组关节间隙狭窄,介于模型组和中药组之间,关节软骨面外观粗糙骨赘增生;中药组关节间隙稍窄,关节面稍粗糙伴轻微骨赘;镜下模型组软骨细胞呈成簇现象增生,中药组软骨细胞大小均匀且排列整齐;TNF-α、i NOS含量模型组中比正常组增高(P<0.01)。两个给药组比模型组明显降低(P<0.01),两给药组之间无差异统计学意义(P>0.05)。结论补肾固筋方有效降低骨关节炎TNF-α、i NOS含量,促进软骨修复,提供实验依据。

Objective To explore the regulation effect of tonifying kidney and consolidating tendon prescription on tumor necrosis factor-alpha( TNF-α) and inducible nitric oxide synthase( i NOS) in knee osteoarthritis( KOA) goats. Methods A total of 72 3-month-old healthy male goats were randomly divided into normal group,model group,Western medicine group,and TCMgroups,with 18 goats in each group. The model was established using modified Hulth method. Serum and the cartilage,synovial membrane,and the subchondral bone of the femur and the medial malleolus were collected. TNF-α and i NOS were detected using ELISA method. Results The joint space narrowed in Western medicine group,and it was between the model group and the TCMgroup. The articular surface became roughness and the osteophyte developed. The joint space slightly narrowed in the TCMgroup,and the articular surface was slightly rough with mild osteophytes. The chondrocytes proliferated in a cluster manner with even size and good order under the microscope. The expression of TNF-α and i NOS was significantly higher in the model group than that in the normal group( P < 0. 01). It was significantly lower in both medicine groups than in the model group( P < 0. 01). The difference between the two medicine groups was not significant( P > 0. 05). Conclusion The tonifying kidney and consolidating tendon prescription effectively decreases the content of TNF-α and i NOS in KOA and promotes the repair of the cartilage. This provides experimental evidence for the clinical treatment.