银杏叶提取物对糖皮质激素诱导的骨质疏松症大鼠骨密度和骨生物力学的影响

Effects of Ginkgo biloba extract on bone mineral density and bone biomechanics in rats with glucocorticoid-induced osteoporosis

目的评估银杏叶提取物(ginkgo biloba extract, Gbe)对糖皮质激素诱导的骨质疏松大鼠血清骨碱性磷酸酶、骨密度、胫骨生物力学的影响。方法经过糖皮质激素诱导骨质疏松后,将大鼠分为五组:骨质疏松组、Gbe-1组、Gbe-2组、阿仑膦酸钠组和对照组。经过4周和8周的治疗,将对照组与骨质疏松组的血清骨碱性磷酸酶、骨密度、胫骨生物力学改变进行比较(Student’s t检验),而骨质疏松组和其他治疗组通过ANOVA检验然后行Tukey/Dunnett’ T3(P<0.05)进行分析。结果在骨质疏松组中,骨碱性磷酸酶、骨密度、骨刚度、最大负荷和弹性降低(P<0.05)。Gbe-1和Gbe-2组骨碱性磷酸酶值增加。此外,Gbe-1,Gbe-2和阿仑膦酸钠组,骨密度增加(P<0.05);与骨质疏松组相比,Gbe-1,Gbe-2和阿仑膦酸钠组显示出骨硬度、最大负荷和弹性值显著增加(P<0.05)。结论银杏叶提取物可显著提高糖皮质激素诱导的骨质疏松大鼠的骨碱性磷酸酶、骨刚度、最大负荷和弹性及骨密度。

Objective To evaluate the effects of Ginkgo biloba extract on bone alkaline phosphatase,bone mineral density and mechanical properties of tibia in rats with glucocorticoid-induced osteoporosis.Methods After osteoporosis induction,the rats were divided into five groups:Osteoporosis;Gbe-1;Gbe-2;alendronate and control.The animals were treated for 4 weeks and 8 weeks.The control group was compared with the osteoporosis group using Student’s t-test,while the osteoporosis group and the other groups were compared using ANOVA test followed by Tukey/Dunnett’T3 post hoc test(P<0.05).Results In the osteoporosis group,bone alkaline phosphatase,bone mineral density,and bone stiffness,maximum load and resilience were reduced(P<0.05).Bone alkaline phosphatase values increased in the Gbe-1 and Gbe-2 groups(P<0.05).In addition,in the Gbe-1,Gbe-2 and alendronate groups,bone mineral density increased.The Gbe-1,Gbe-2 and alendronate groups showed a significant increase in bone stiffness,maximum load and resilience values when compared with the osteoporosis group.Conclusion The extract of Ginkgo biloba restored bone alkaline phosphatase and bone stiffness,maximum load and resilience,as well as bone mineral density measured using dual-energy X-ray absorptiometry in rats with glucocorticoid-induced osteoporosis.