白屈菜内生真菌的分离鉴定及其代谢产物的抗真菌活性研究

Isolation and identification of endophytic fungi from Chelidonium majus and their antifungal activity

为寻找新型抗真菌活性物质的来源,该研究采用常规真菌分离方法,从药用植物白屈菜的根、茎及叶中共分离得到11株可培养的内生真菌,结合分子生物学与形态学鉴定了其中10株,其中刺盘孢属Colletotrichum 7株(L1,L2,L3,S1,S3,S4,S5),镰刀菌属Fusarium 3株(R1,R2,R3)。抗真菌活性测试结果表明,所有菌株代谢产物对5种常见植物病原菌(苹果炭疽菌、玉米弯孢菌、水稻稻瘟菌、烟草赤星菌和白菜黑斑菌)均表现出一定程度的抗菌活性,部分测试项目抑制率超过60%,其中菌株R1,S2,S3,S4的活性相对较高。该研究丰富了对白屈菜内生菌的认识,同时为新型微生物源抗菌物质的深入研究奠定了基础。

In order to find new source of antifungal agents, eleven cultivable endophytic fungi were isolated from the roots,stems and leaves of Chelidonium majus by traditional method. Seven of them were identified as Colletotrichum(L1, L2, L3, S1, S3, S4, S5), and three of them were identified as Fusarium(R1,R2,R3) by morphological features and molecular biological technology. The antifungal activity test showed that all the tested fungi displayed some inhibitory activity against five common plant pathogens(C. gloeosporioides, Curvularia lunata, Pyricularia oryza, Alternaria alternate and A. brassicae), and their inhibition rate of some test items were over 60%. Among them, R1, S2, S3 and S4 were more potent than others. This study enriches the understanding of endophytes from Ch. majus and provides a basis for the study of new microbial fungicides.