外源激素IBA和真菌诱导子对八角莲离体根生长及其鬼臼毒素合成的影响

Effects of exogenous IBA and fungal elicitor on growth of in vitro roots culture of Dysosma versipellis and production of podophyllotoxin

该实验以White为基本培养基,研究了不同浓度外源激素IBA和真菌诱导子对八角莲离体根生长和次生代谢产物鬼臼毒素合成的影响。结果表明,经过3周的培养,生长激素IBA和内生真菌Zasmidium syzygii诱导子均能提高八角莲离体根中鬼臼毒素的含量;在添加3 mg·L-1 IBA的White培养基中,离体根中鬼臼毒素的质量分数最高为1 830.86μg·g-1,是对照组的2.07倍;其次是添加1.5 mg·L-1 IBA,Z.syzygii真菌诱导子,1,0.5,4.5 mg·L-1 IBA,鬼臼毒素的含量分别是对照组的1.82,1.71,1.63,1.43,1.1倍。离体根生长与IBA浓度的变化呈一定的正相关性。上述结果初步说明,最适于八角莲离体根代谢产生鬼臼毒素的IBA质量浓度为3 mg·L-1;Z.syzygii真菌诱导子的促进作用效果介于1.5 mg·L-1和1 mg·L-1的IBA之间,是将来生产中诱导八角莲中鬼臼毒素积累的潜在天然诱导子。

Using the White as basic medium,the effects of the exogenous IBA and endophytic fungal elicitor on the growth of in vitro roots cultures of Dysosma versipellis and production of podophyllotoxin were investigated in this study.The results showed that the IBA and the endophytic fungus Zasmidium syzygii elicitor could increase the content of podophyllotoxin of in vitro roots of D.versipellis after 3 weeks.The White medium added with 3 mg·L-1 IBA induced the highest increase of podophyllotoxin(1 830.86μg·g-1),which was 2.07 folds greater than the control,and followed by 1.5 mg·L-1 IBA,fungal elicitor,1 mg·L-1 IBA,0.5 mg·L-1 IBA and 4.5 mg·L-1 IBA,which was 1.82,1.71,1.63,1.43 and 1.1 folds greater than the control,respectively.The results also showed that the growth of roots was certain positively correlated with the change of IBA concentration.Therefore,3 mg·L-1 IBA was the most suitable for the production of podophyllotoxin in the in vitro roots of D.versipellis,and the stimulating effect of Z.syzygii fungal elicitor was between 1.5 mg·L-1 and 1 mg·L-1 IBA,which was a potential natural elicitor to induce the accumulation of podophyllotoxin in future production.