新鲜和干燥当归干预血虚大鼠的补血作用及谱效关系研究

Spectrum-effect relationships on enriching blood activities of fresh and dry roots of Angelica sinensis

采用液相色谱质谱(LC-MS)方法建立10个不同产地的新鲜当归(FRAS)及干燥当归(RAS)色谱指纹图谱;用乙酰苯肼(APH)和环磷酰胺(CPX)联合复制血虚大鼠模型,观察FRAS和RAS对血虚大鼠生理指标的影响。通过灰色关联度分析(grey relational analysis,GRA)和偏最小二乘(partial least squares,PLS)回归分析法将LC-MS色谱指纹图谱中各共有峰的相对含量和补血药效数据相关联,建立谱效关系。结果显示,FRAS和RAS均有一定的补血作用(P<0.05)。根据PLS回归系数大小确定了FRAS和RAS中各共有峰对补血作用的贡献程度。其中,4个色谱峰对补血作用的贡献较大,分别为P1(未知),P2(未知),P7(阿魏酸),P11(洋川芎内酯A)。该实验研究了FRAS和RAS的LC-MS色谱指纹图谱与补血作用之间的相关性,确定了二者中的补血药效成分,为当归资源的合理应用提供了一定的理论基础。

Ultra performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(LC-MS)was used to establish the chromatography fingerprint for fresh(FRAS)and dry(RAS)roots of Angelica sinensis from 10 different places.The rat model of blood deficiency was established by acetyl-phenyl-hydrazine(APH)and cyclophosphamide(CTX).Then grey relational analysis(GRA)and partial least squares regression(PLS)were used to investigate the spectrum-effect relationship between the relative contents and the data of enriching blood pharmacodynamics efficacy.The results showed that the FRAS and RAS had certain enriching blood activities(P<0.05).The contribution degree of the FRAS and RAS to enriching blood activities of each common peaks were determined by regression coefficient.Among them,4 common peaks contributed significantly to the effect of enriching blood activities,P1(unknown),P2(unknown),P7(ferulic acid),and P11(senkyunolide A)respectively.This paper investigated the spectrum-effect relationship between enriching blood activities and LC-MS chromatography fingerprint of RAS and FRAS,and determined the effective compositions of RAS and FRAS with enriching blood activities.It lays a theoretical foundation for the comprehensive development and utilization of A.sinensis.