当归不同炮制品的UPLC指纹图谱与多成分含量测定研究

UPLC fingerprint and multi-components content determination of different processed products of Angelica sinensis

选择来源于甘肃驮乡、岷县、渭源3个产地的10批当归为研究对象,通过规范的炮制方法分别炮制成生当归、酒当归、土当归。采用UPLC建立当归3种炮制品的指纹图谱,并对其中9种酚酸及苯酞类成分进行指认。采用《中药色谱指纹图谱相似度评价系统》进行相似度分析,结果显示当归同一炮制品样品色谱峰基本相似,相似度均大于0. 950;不同炮制品与其对照图谱之间差别不明显,相似度亦均大于0. 950。在采用主成分分析和正交偏最小二乘判别分析,寻求组间差异性成分的基础上,采用开发的改良距离系数法,可有效的通过指纹图谱相似度对生当归、酒当归、土当归进行区分。同时采用UPLC建立了当归9种酚酸及苯酞类成分含量测定方法,并进行了不同炮制品之间的比较。实验结果显示与生当归相比,各成分均有变化,其中酒当归中阿魏酸松柏酯及藁本内酯含量明显上升;当归土炒之后阿魏酸松柏酯明显上升。该实验建立的方法可有效地对当归不同炮制品进行区分,同时对其中主要成分进行含量测定。

Ten batches of Angelica sinensis from three producing areas(Tuoxiang,Minxian and Weiyuan of Gansu province)were selected as the research objects,and processed into raw A.sinensis,A.sinensis with alcohol,and A.sinensis with soil respectively through the standard processing methods.Ultra-high performance liquid chromatography(UPLC)was used to establish fingerprint for three processed products of A.sinensis,and determine the contents of 9 phenolic acids and phthalide compounds.The similarity was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine,which showed that the chromatographic peaks of the same processed samples of A.sinensis were basically similar,with all similarities greater than 0.950.The difference between different processed products and their control spectra was not obvious,with all similarities also higher than 0.950.On the basis of using principal component analysis(PCA)and OPLS-DA to seek the difference components between groups,the improved distance coefficient method can be used to effectively distinguish the three processed products of A.sinensis by fingerprint similarity.At the same time,the determination method of nine phenolic acids and phthalide in A.sinensis was established by UPLC,and the comparison between different processed products was carried out.The results showed that the content of various components was changed as compared with the raw A.sinensis.The contents of coniferyl ferulate and ligustilide in the A.sinensis with alcohol were increased significantly,and the content of coniferyl ferulate was obviously increased in A.sinensis with soil.The method established in this paper can effectively distinguish different processed products of A.sinensis and determine the content of the main components in them.