摘要
采集了3个不同主产区32批种植或野生乌拉尔甘草药材,采用指纹图谱、LC-TOF/MS及HPLC多指标成分分析结合模式识别(PCA和OPLS-DA)对不同主产地甘草样品水溶性特征组分进行差异性分析。32批次药材指纹图谱相似度在0. 903~0. 999,相似度较高;采用模式识别可以将甘肃、新疆种植药材与内蒙古种植药材及野生药材进行区分,进一步以LCTOF/MS及对照品对照鉴定了指纹图谱中31个共有峰成分,并对其中4个黄酮苷及5个三萜皂苷特征组分含量进行了对比分析,结果表明3个产区种植甘草药材中5个三萜皂苷总含量差异不明显,但4个黄酮苷总含量呈现出内蒙古>甘肃≈新疆的趋势(P<0. 05),而野生药材与种植药材相比4个黄酮苷及5个三萜皂苷总含量均有显著提升(P<0. 01);其中,甘肃与新疆种植药材在甘草苷、异甘草苷、甘草皂苷A3、22β-乙酰甘草酸及乌拉尔皂苷B上的含量均要低于内蒙古地区种植药材,但主要指标性成分甘草酸在3个产区药材中含量差异并不明显,而在内蒙古产区内的野生药材比种植药材在甘草苷、异甘草苷、甘草皂苷A3、甘草皂苷G2及甘草酸含量上均有显著升高。多指标成分定性/定量分析结合模式识别能有效评价不同区域种植及野生甘草的质量,有助于深入了解不同区域甘草的现状,可为甘草的资源开发与综合利用提供科学依据。
Thirty-two batches of cultivated and wild Glycyrrhiza uralensis were obtained from three geographical regions.Comparative study of water characteristic components of G.uralensis from three geographical origins was conducted by PCA,OPLS-DA chemical pattern recognition combined with LC-TOF/MS and muti-component analysis.The similarity of fingerprints of 32 batches of medicinal materials ranged from 0.903 to 0.999.Patterns recognition could be used to distinguish cultivated G.uralensis in Gansu and Xinjiang areas from cultivated and wild plants in Inner Mongolia.Then a total of thirty-one common constituents were identified by LC-TOF/MS analysis coupled with standard compounds information.The contents of four flavonoid glycosides and five saponins were determinated by HPLC and compared using One-way ANOVA.The results showed that there was no significant difference in the contents of 5 triterpenoid saponins among the three regions,but the contents of 4 flavonoid saponins showed the trend of Inner Mongolia>Gansu≈Xinjiang(P<0.05).In the same Inner Mongolia region,the contents of 4 flavonoid glycosides and 5 triterpenoid saponins in wild plant was significantly higher than that in cultivated plants(P<0.01).In addition,the contents of liquiritin,isoliquiritin,licorice-saponin A3,22β-acetoxyl-glycyrrhizic acid and uralsaponin B in Gansu and Xinjiang were obviously lower than those in Inner Mongolia,but the contents of glycyrrhizic acid,the main component of G.uralensis,were not different in the three geographical regions.In Inner Mongolia,the contents of liquiritin,isoliquiritin,licorice-saponin A3,licorice-saponin G2 and glycyrrhizic acid in wild plants were significantly higher than those in cultivated plants.In conclusion,qualitative/quantitative analysis of multi-index components combined with pattern recognition could effectively evaluate the quality of cultivated and wild licorice in different regions.It was helpful for us to understand the reality of licorice in different regions,and provided scientific basis for the development and comprehensive utilization of licorice resources.
作者
郑云枫
段伟萍
杨阳
许志宇
李存玉
陈丽红
彭国平
ZHENG Yun-feng;DUAN Wei-ping;YANG Yang;XU Zhi-yu;LI Cun-yu;CHEN Li-hong;PENG Gou-ping(School of Pharmacy,Nanjing University of Chinese Medicine,Nanjing 210023,China;Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization,Nanjing 210023,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2019年第12期2544-2551,共8页
China Journal of Chinese Materia Medica
基金
国家重点研发计划项目(2018YFC1706503)
国家中药标准化项目(ZYBZH-J-JS-34)