不同来源白及中3个主要化学成分的含量比较

Comparison of contents of three active ingredients in Bletilla striata from different sources

为了了解相同种植条件下60个不同来源白及中贝母兰宁、山药素Ⅲ和3’-O-甲基山药素Ⅲ的含量差异。该研究采用UPLC进行测定,采用Waters ACQUITY UPLC BEH C18(2. 1 mm×100 mm,1. 7μm)色谱柱,以乙腈-0. 1%甲酸水为流动相进行梯度洗脱,流速为0. 208 m L·min-1,检测波长270 nm,柱温35℃,进样量为4μL。结果表明,在上述色谱条件下3个成分分离度良好,在0. 156~5. 000 mg·L-1线性良好,测得贝母兰宁、山药素Ⅲ和3’-O-甲基山药素Ⅲ平均质量分数分别达(0. 116±0. 071)%,(0. 386±0. 185)%,(0. 086±0. 034)%。不同来源、不同品种白及在相同条件下种植2年后,化学组成差异不大,但3个成分含量具有一定的地域性差异,表现为西部地区高于东部地区的趋势;而华白及中贝母兰宁、山药素Ⅲ含量稍高于白及。该色谱方法简单,稳定性、重复性好,可用于3个成分的定量分析;不同来源白及成分含量分析可为今后白及优良品种选育和质量标准制定提供参考。

In order to understand the difference of contents of coelonin,batatasinⅢand 3’-O-methylbatatasinⅢin 60 different sources of Bletilla striata planted under the same conditions.UPLC method was used and the analysis was performed on a Waters ACQUITY UPLC BEH C18 column(2.1 mm×100 mm,1.7μm),eluted with acetonitril-0.1%formic acid solution by gradient.The flow rate was 0.208 m L·min-1,the detection wavelength was 270 nm,the column temperature was 35℃and the injection volume was 4μL.Under the above chromatographic conditions,the three components can be separated well with good linearity in the range of 0.156-5.000 mg·L-1.The average contents of coelonin,batatasinⅢand 3’-O-methylbatatasinⅢwere(0.116±0.071)%,(0.386±0.185)%and(0.086±0.034)%,respectively.After planting for two years under the same conditions,there was no significant difference in chemical composition among different sources and varieties,but the contents of the three components had some regional differences,which indicated that the western region was higher than the eastern region,while the contents of coelonin and batatasinⅢin B.sinensis were slightly higher than those in B.striata.The chromatographic method above is simple,stable and reproducible,and can be used for quantitative analysis of three components.The content analysis of different sources of B.striata can provide reference for future B.striata breeding and quality control.