菝葜UPLC指纹图谱与其抗大鼠盆腔炎作用的谱效关系研究

Spectrum-effect relationship between UPLC fingerprint of Smilax china and anti-pelvic inflammation in rats

研究菝葜UPLC指纹图谱与其抗盆腔炎作用的相关性,探究菝葜抗盆腔炎的药效物质基础。建立10批不同产地菝葜UPLC指纹图谱,测定盆腔炎模型大鼠的SOD,MDA,TNF-α,IL-6,采用层次分析法确定各单一指标对总药效的权重,运用灰色关联度法分析菝葜指纹图谱中各峰对各单一药效指标及总药效的贡献,并通过与对照品比对指认色谱峰化学成分结构。10批菝葜UPLC指纹图谱27个共有特征峰均是与菝葜抗盆腔炎症作用相关色谱峰,其中13个色谱峰被指认,分别为2号峰(3,5-二羟基-2-甲基苯甲酸-3-O-葡萄糖苷),3号峰(绿原酸),5号峰(2,7,4’-三羟基黄酮-5-O-葡萄糖苷),6号峰(二氢山柰酚-5-O-葡萄糖苷),7号峰(二氢山柰酚-7-O-葡萄糖苷),9号峰(花旗松素),10号峰(白藜芦醇苷),11号峰(氧化白藜芦醇),12号峰(落新妇苷),15号峰(白藜芦醇),16号峰(槲皮苷),18号峰(黄杞苷)以及24号峰(山柰酚);有21个色谱峰与总药效的关联度大于0. 8,按关联度排名前10的顺序为1,3,7,19,18,17,4,11,16,21号峰。研究结果显示,菝葜抗盆腔炎为药效物质群共同作用的结果;为了更有效控制菝葜药材及饮片质量,应合理选择含量检测的指标性成分。

To study the correlation between ultra high performance liquid chromatography(UPLC)fingerprint of Smilax china and its anti-pelvic inflammatory effect,and to explore the pharmacodynamic material basis of S.china against pelvic inflammatory disease.UPLC fingerprints of 10 batches of S.china from different habitats were established,and the values of SOD,MDA,TNF-α,and IL-6 in rats with pelvic inflammation were measured.The weight of each single pharmacodynamics index to the total efficacy was determined by analytic hierarchy process,and the contribution of each peak in fingerprints to the each single pharmacodynamics index and total efficacy was analyzed by the grey relational analysis.Then the structures of chemical constituents at the identified peaks were confirmed by comparing with the reference substance.The 27 common characteristic peaks of UPLC fingerprints were all related to the anti-pelvic inflammation effect of S.china,of which 13 peaks were identified as peak 2(3,5-dihydroxy-2-methylbenzoic acid-3-O-glucoside),peak 3(chlorogenic acid),peak 5(2,7,4-trihydroxydihydroflavone-5-O-glucoside),peak 6(7,4-dihydroxydihydroflavonol-5-O-glucoside),peak 7(taxifolin-7-O-glucoside),peak 9(taxifolin),peak 10(polydatin),peak 11(oxyresveratrol),peak 12(astilbin),peak15(resveratrol),peak 16(quercitrin),peak 18(engeletin)and peak 24(kaempferol).The correlation degree of 21 peaks and the total efficacy was greater than 0.8,and the top 10 ranked by correlation degree were as follows:peak 1,3,7,19,18,17,4,11,16,and 21.The results showed that the anti-pelvic inflammation effect of S.china was achieved by the combined action of pharmacodynamic substances.In order to control the quality of S.china and its prepared slices more effectively,the index components of content detection should be selected reasonably.