基于DNA条形码、UPLC及色度学方法鉴定与评价化橘红

Identification and evaluation of Citrus grandis based on DNA barcode,UPLC and chromaticity method

为鉴定化橘红的来源,并对2个地区化橘红的质量进行综合性评价,该研究采用DNA条形码对26份化橘红进行分子鉴定;以柚皮苷、野漆树苷、柚皮素和芹菜素为指标性成分采用UPLC进行含量测定,采用色度仪对化橘红颜色差异进行数值化研究,并与其有效成分进行关联性分析。结果表明2个地区的化橘红样品来源均为柚Citrus grandis,ITS2序列长度均为400~500 bp,序列相似度达到99. 82%,2组样品仅有1个碱基的缺失,即在330 bp处广东的部分药材多一个碱基A,而重庆地区样品无此碱基;重庆地区的样品柚皮苷与野漆树苷的含量高于广东地区的样品,柚皮素与芹菜素的含量也有着统计学差异;色度值结果显示广东的药材L*偏大,a*偏小,重庆的药材L*偏小,a*偏大,而两者的b*则无明显的差异;相关性分析结果表明柚皮苷、野漆树苷、柚皮素与L*,b*呈正相关,与a*呈负相关,芹菜素与L*,a*,b*值无相关性。该研究对化橘红进行科学的鉴定与评价,为进一步研究化橘红的快速鉴别与质量评价提供新的思路。

In order to identify the source of Citrus grandis and evaluate its quality originate from two areas comprehensively,DNA barcode was used to identify 26 samples of C.grandis.The content of naringin,rhoifolin,naringenin and apigenin was determined by UPLC method,and the color difference was numerically studied by color difference analyzer,which was related to the effective components of C.grandis.The results showed that samples was the source of C.grandis in both regions.The ITS2 sequence length was about400-500 bp,and the sequence similarity reached 99.82%.There was only one base deletion in the two groups.There was one base A in some medicinal materials of Guangdong at 330 bp,but no base in Chongqing.The contents of naringin and rhoifolin in Chongqing samples were higher than those in Guangdong samples,and there were statistical differences between naringenin and apigenin.The chroma value showed that L*value of Guangdong was larger,a*value was smaller,L*value of Chongqing was smaller,and a*value was larger,while the b*value of both was not significantly different;The results of correlation analysis showed that naringin,rhoifolin,naringenin were positively correlated with L*,b*value,negatively correlated with a*value,and apigenin had no correlation with L*,a*,b*value.In this study,the scientific identification and evaluation of C.grandis was carried out to provide a new idea for the further study of the rapid identification and evaluation of C.grandis.