黄芩炭对早孕流产大鼠子宫出血保护作用及机制研究

Research on hemostatic effect and mechanism of carbonized Scutellariae Radix on rats of uterine bleeding

研究黄芩炭对早孕流产大鼠子宫出血保护作用及其机制。取未怀孕的雌性大鼠8只作为正常组,同一天受孕的雌性大鼠40只,以米非司酮(11. 4 mg·kg-1)和米索前列醇(125μg·kg-1)建立大鼠早孕不完全流产模型,将流产模型大鼠分为模型组,黄芩炭水提液低(0. 55 g·kg-1)、中(1. 10 g·kg-1)、高(2. 20 g·kg-1)剂量组及阳性药断血流片(0. 45 g·kg-1)组。紫外分光光度法测定大鼠子宫出血量,HE染色法观察子宫内膜病理变化,ELISA法测定血浆中IL-1β,IL-6和TNF-α水平,RT-PCR法检测子宫组织中IL-1β,IL-6和TNF-αmRNA水平,Western blot法检测子宫组织中VEGF,MMP-2,MMP-9蛋白表达量。结果显示,与正常组相比,模型组大鼠子宫出血量显著增加,病理评分显著升高,微血管密度明显减少,血浆中TNF-α和IL-1β含量均显著升高,IL-6含量显著降低,TNF-α和IL-1βmRNA表达显著升高,IL-6 mRNA表达显著降低,大鼠子宫VEGF,MMP-2,MMP-9表达量显著升高;与模型组相比,黄芩炭中、高剂量组可显著降低流产大鼠子宫出血量,促进子宫内膜修复及升高子宫内膜组织微血管密度,降低大鼠子宫内TNF-α和IL-1β的含量及mRNA表达量,升高IL-6的含量及mRNA表达量,降低大鼠子宫VEGF,MMP-2,MMP-9等蛋白的表达。表明黄芩炭具有止血作用,其止血机制可能与修复子宫内膜、抑制炎症反应有关。

The aim of this paper was to investigate the hemostatic effect and mechanism of carbonized Scutellariae Radix on uterine bleeding in the rats caused by early pregnancy termination.Eight unpregnant female rats were selected as normal group.Forty female rats conceived on the same day received mifepristone(11.4 mg·kg-1)and misoprostol(125μg·kg-1)to induce model of incomplete abortion in early pregnancy.Abortion models were randomly divided into model group,carbonized Scutellariae Radix water extract low dose group(0.55 g·kg-1),medium group(1.10 g·kg-1),high dose group(2.20 g·kg-1)and positive control group(0.45 g·kg-1).The uterine bleeding volume was detected by ultraviolet spectrophotometry.The pathological changes of endometrium were detected by HE(hematixylin-eosin)staining.The levels of interleukin(IL-1β),IL-6 and tumor necrosis factor(TNF-α)in the plasma of rats were determined by ELISA.The expression levels of IL-1β,IL-6 and TNF-αmRNA in the uterus of rats were determined by RT-PCR.The protein expression levels of VEGF,MMP-2 and MMP-9 were determined by Western blot.As compared with the normal group,the uterine bleeding volume and histopathological score were increased significantly;microvessel density of endometrial tissues was decreased significantly;the contents of TNF-α,IL-1βand the levels of TNF-αmRNA and IL-1βmRNA in the plasma were increased,while the content of IL-6 and level of IL-6 mRNA were decreased significantly.The protein expression levels of VEGF,MMP-2 and MMP-9 in the uterine tissues were also decreased.As compared with the model group,the uterine bleeding volume was decreased significantly in the carbonized Scutellariae Radix medium dose and high dose groups;endometrial repair was promoted,and the microvessel density of endometrial tissues was increased significantly;the contents of IL-1βand TNF-αin the plasma of rats were decreased significantly,while the content of IL-6 in the plasma of rats was increased significantly;the expression levels of IL-1βand TNF-αmRNA in the uterus of rats were decreased and the expression level of IL-6 mRNA showed an increase;the protein expressions of VEGF,MMP-2 and MMP-9 were decreased significantly in carbonized Scutellariae Radix medium and high dose groups.In conclusion,carbonized Scutellariae Radix showed good hemostatic effect,and its mechanism may be related to the repair of endometrium and inhibition of inflammatory reaction.