基于数据挖掘的藏族医治疗HAPC高频次使用藏族药网络药理学研究

Network pharmacology research on high frequency use of Tibetan medicine in treatment of HAPC based on data mining

该研究基于前期数据挖掘结果,采用网络药理学方法,分析藏族医治疗高原红细胞增多症(HAPC)高频次使用藏族药作用机制。作者以前期数据挖掘获得藏族医治疗HAPC高频次使用藏族药诃子、木香、西红花、肉豆蔻为研究对象;综合文献及TCMSP数据库分别筛选出每味高频次藏族药前5个主要活性成分,经PharmMapper及Drug Bank数据库筛选出各味药潜在的作用靶点,将得到靶点分别导入MAS 3. 0数据库获取相应通路信息,结合KEGG数据库进行通路注释及GO功能富集分析,最后采用Cystoscope 3. 4. 0软件分别构建4味高频藏族药'化合物-靶点-通路'网络图。其中4味药与HAPC相关对应的靶点数为诃子16个、木香20个、西红花20个、肉豆蔻15个,共有靶点为BHMT,F2,ADH5,AKR1C2,GSK3B,INSR,PDE4B;涉及通路有诃子17条、木香17条、西红花24条、肉豆蔻14条,共有通路为metabolism of xenobiotics by cytochrome P450。结果表明,藏族医治疗HAPC高频次藏族药诃子、木香、西红花、肉豆蔻具有共同的通路及靶点,其可能都是通过介导机体细胞繁殖和氧化应激、发挥抗炎作用、参与调节血管等功能,达到降低血红蛋白、增强机体免疫力的效果从而发挥治疗HAPC的作用。该研究从信息化层面剖析了藏族医药防治HAPC的多成分、多靶点、多通路的作用机制,为藏族医药多维视野下防治高原病深入研究提供有利参考。

Based on the results of previous data mining,the mechanism of high frequency use of Tibetan medicine in the treatment of high altitude polycythemia(HAPC)was analyzed in this study by network pharmacology.The author obtained the high frequency use data on Tibetan medicine Terminalia chebula,Aucklandia lappa,Crocus sativus and Myristica fragrans for the treatment of HAPC by data mining in the previous period.The first five main active ingredients of each high frequency Tibetan medicine were screened out by reviewing comprehensive literature and TCMSP database.The potential targets of each medicine were screened by PharmMapper and Drug Bank database,and then the targets were imported into MAS 3.0 database to obtain the corresponding path information.The KEGG database was used for path annotation and GO function enrichment analysis.Finally,Cystoscope 3.4.0 software was used to construct'compound-target-path'network for four high-frequency Tibetan medicines.Among them,the target points of four herbs related to HAPC were 16(T.chebula),20(A.lappa),20(C.sativus),and 15(M.fragrans).The common target points included BHMT,F2,ADH5,AKR1 C2,GSK3 B,INSR and PDE4 B,involving pathways related to T.chebula(17),A.lappa(17),C.sativus(24)and M.fragrans(14),and the common pathway was metabolism of xenobiotics by cytochrome P450.The results showed that high-frequency Tibetan medicine had common pathways and targets in treating HAPC,such as T.chebula,A.lappa,C.sativus and M.fragrans.The medicines could reduce hemoglobin and enhance immunity by mediating cell proliferation and oxidative stress,exerting anti-inflammatory effects and participating in regulating blood vessels,showing therapeutic effects for HAPC.In this study,the multi-component,multi-target and multi-pathway mechanism of Tibetan medicine in preventing and treating HAPC was analyzed from the information level,providing a useful reference for further study of Tibetan medicine in preventing and treating plateau diseases from the multi-dimensional perspective.