RP-HPLC法测定慢肝养阴颗粒中五味子醇甲的含量

Determination of Schizandrol A in ManGanYangYin Granules by HPLC

目的建立慢肝养阴颗粒中五味子醇甲的含量测定方法。方法采用RP-HPLC法,以甲醇-水(63:37)为流动相;检测波长为250nm。结果五味子醇甲在10.24-153.60μg·mL-1(r=1.0000)范围内线性关系良好;平均回收率为99.85%,RSD为0.54%(n=6)。结论本文方法简便可靠,专属性强,重现性好,可用于慢肝养阴颗粒的质量控制。

Objective To establish the Determination of Schizandrol A in ManGanYangYin Granules.MethodsHPLC wasused and methanol-water(63:37) mixture as a mobile phase.The detection wavelength was set at 250nm.Results Schizandrol A showed a good linear relationship at a ranger of 10.24-153.60μg·mL-1,r =1.0000.The average recovery was 99.85%,and RSD was 0.54%(n =6).Conclusion The method is simple with good reproducibility and can be used ManGanYangYin Granules.