微量离心柱法检测AFP-L3的临床应用

Microcentrifuge column method detects the clinical application of AFP-L3

目的本文旨在探讨微量离心柱法检测甲胎蛋白异质体(AFP-L3)在肝癌预警及良恶性肝病鉴别诊断中的临床应用价值。方法应用装有耦联小扁豆凝集素(LCA)的微量离心柱分离200例肝病患者的AFP-L3,采用化学发光法检测AFP及AFP-L3,计算AFP-L3占总AFP的比率(判断标准以AFP-L3%≥10%者为阳性)。结果①原发性肝癌组AFP-L3(%)均值和阳性率明显高于肝硬化组和慢性肝炎组(P【0.001);低浓度AFP(AFP【200ng/ml)原发性肝癌组AFP-L3(%)经过临床检验也明显高于肝硬化组和慢性肝炎组(P【0.01)。②在原发性肝癌组中,比较不同浓度的AFP(%)均值和阳性率,相互间的差异值P】0.05,在统计学上没有意义。结论经过临床研究,我们认为微量离心柱法检测甲胎蛋白异质体(AFP-L3)在肝癌细胞诊断、预警及与良恶性肝病的鉴别诊断中具有重要的临床应用价值。

Objective To explore the clinical value of Lens culinaris agglutinin-reactive diagnosis between benign and malignant liver diseases,as well as the early warning of hepatocellular carcinoma.Methods lpha-fetoprotein variants from 200 patients with liver diseases were isolated with micro-spin column equipped lens culinaris agglutinin(LCA).The AFP and AFP-L3 were detected by the electrochemical luminescence(ECL)method,and the proportions of AFP-L3 were calculated.Results The positive rate and the mean level of AFP-L3 either in the patients with primary liver cancer or in the patients with primay live cancer whose AFP(<200ng/ml)were statistic significantly different from the patients with chronic hepatitis or cirrhosis of liver(p<0.001).However,in the groups of primary liver cancer,there was no significant difference of the serum mean level and the positive rate of AFP-L3 among the groups of different AFP concentration(P>0.05).Conclusions The detection of AFP-L3 by micro-spin column assay show great clinical value in the differentiation diagnosis of benign and malignant liver diseases,as well as the early waming of,hepatocellular Carcinoma.