HPLC法同时测定保健食品中黄豆黄苷以及黄豆黄素的含量

为了建立同时测定保健食品中黄豆黄苷、黄豆黄素两种成分的方法。我们将色谱柱设定为AgilentzorbaxSB-C18(4.6mm×150mm,5μm),流动相通过乙腈-0.1%甲酸对其梯度洗脱,并将检测波长设定成255nm,流速为1mL·min-1。通过实验我们测出黄豆黄苷、黄豆黄素的线性范围以及加样回收率,并且使用此方法进行实检测操作起来较为简单,测定结果也较为精确,可将此方法在保健食品中黄豆黄苷、黄豆黄素两种成分的含量测定以及产品质量控制中多加应用。

In order to Create a simultaneous determination of the method of the health food glycitin,glycitein,two ingredients.The column is set to Agilent ZORBAX SB-C18(4.6 mm × 150 mm,5 μm),acetonitrile-0.1% formic acid mobile phase through its gradient elution and detection wavelength was set to 255nm,the flow rate was 1mL·min-1.Experimentally measured glycitin,the glycitein linear range and recovery,and the real detection using this method is relatively simple to operate,more accurate measurement results,this method can be health food soy yellow glycosides,glycitein Determination of two components,as well as product quality control in more applications