受激发损耗(STED)显微术及在生物邻域的应用

An Introduction on Stimulated Emission Depletion(STED) Microscopy and Its Bilogical Application

受激发射损耗(STED)显微成像通过引入一束损耗光以受激发射的方式减小有效荧光的发光面积,激发光激发样品的荧光基团形成一个艾里斑,损耗光生成中空型的面包圈抑制艾里斑周围的荧光强度,使我们仅看到中心的强度。基于StefanW.Hell第一次提出受激发射损耗显微术,本文介绍了受激发射损耗(STED)显微术的发展过程,并阐述了近几年来,STED在生物领域的应用。最后,总结和展望了该技术的应用前景。

Stimulated emission depletion(STED) microscopy can reduce the effective area of the fluorescent emission in the excited manner so that we only see the intensity of the center through introducing an additional depletion light beam,and the excitation light excite samples form a fluorophore Airy,depletion light beam generate hollow donut suppress fluorescence intensity around the Airy disk. This paper introduces the development process of stimulated emission depletion(STED) microscopy and also elaborates STED applications in biological field in recent years,based on STED microscopy first proposed by Stefan W. Hell,with a summary and prospect of the application of this technology.