喉癌TU177细胞系中CD133^+CD44^+肿瘤干细胞分选及特性分析

Biological Characteristics of CD133^+CD44^+ Cancer Stem Cells Sorting from Laryngeal Carcinoma Cell Line TU177

目的:免疫磁珠分选喉癌TU177细胞系中的CD133^+CD44^+细胞,探讨CD133^+CD44^+细胞作为肿瘤干细胞的生物学特性。方法 :培养喉癌TU177细胞,采用免疫磁珠分选技术分选CD133^+CD44^+、CD133^+CD44~^-、CD133~^-CD44^+、CD133~^-CD44~^-细胞,流式检测分选效率,检测各组细胞的增殖、侵袭、迁移、粘附、克隆形成能力。结果:CD133^+CD44^+细胞的增殖、迁移、侵袭、粘附、克隆能力均明显高于其他组,差异有统计学意义(P<0.0001)。结论:免疫磁珠技术能有效进行TU177细胞系的分选,CD133^+CD44^+细胞亚群具有强增殖、侵袭、迁移、粘附、克隆形成能力,具有肿瘤干细胞特征,CD133作为干细胞标志物,其干细胞特性强于CD44,可为喉癌的进一步靶向治疗提供依据。

Objective :Magnetic activated cell sorting was used to separate CD133^+CD44^+ cancer cells from laryngeal carcinoma TU177 cell line. Analysis the biological characteristics of these subpopulations.Methods :TU177 cells were subjected to magnetic activated cell sorting to obtain CD133^+CD44^+、CD133^+CD44^-、CD133^-CD44^+、CD133^-CD44^- cells. Evaluate the efficiency of magnetic separation by flow cytometry. Test cell proliferation,migration,invasion,adhesion,colony forming ability of the cells.Results: CD133^+CD44^+ cells show higher proliferation,migration,invasion,adhesion,clone ability than other group(P<0.0001).Conclusions:TU177 cells can be serparated by Magnetic activated cell sorting effectively. CD133 is more powerful than CD44.Our study may provide evidence for target treatment of laryngeal cancer.