浅析间接免疫荧光法筛查抗核抗体与特异性抗体检测的相互关系

Analysis of the Relationship between the Detection of Anti Nuclear Antibodies and Specific Antibodies by Indirect Immunofluorescence

目的研究间接免疫荧光法筛查抗核抗体与特异性抗体检测的相互关系。方法选取我院在2015年8月-2016年1月间680例送检血清标本的抗核抗体,将所有标本分为A、B、C 3个小组,其中A组为自身免疫性疾病(AID)的标本,B组为疑似AID标本,C组为非AID标本;所有标本均采用间接免疫荧光法(IIF)进行筛查,并通过线性免疫印迹法(LIA)检测特异性抗体,观察比较两种检测方法的检测结果。结果所有标本通过检测后显示,两种方法均表现为阳性的标本有304例(44.71%),均表现为阴性的有173例(25.44%),两种方法的抗核抗体检测符合率为(70.15%)。结论抗核抗体采用IIF进行筛查易造成一些特异性抗体出现漏诊,而只检测特异性抗体,易造成抗核抗体漏诊,应给予患者综合检测。

Objective To study the relationship between the detection of anti nuclear antibodies and specific antibodies by indirect immunofluorescence. Methods all specimens were divided into A, B, C three groups, Among them, group A is the specimen of autoimmune disease(AID), B group is suspected AID specimen, C group is non AID specimen; all specimens are used indirect immunofluorescence(IIF) screening, and through linear immunoassay(LIA) to detect the specific antibody were observed on the results of the two detection methods were compared. Results all specimens through testing show that two methods showed positive samples in 304 cases(44.71 %), 173 cases were negative(25.44 %), and the detection rate of antinuclear antibodies in two methods was(70.15 %). Conclusion It is easy to make some specific antibodies by IIF to screen the anti nuclear antibody, and only the specific antibody is detected, which is easy to cause the missed diagnosis of anti nuclear antibodies.