研究不同标本对卡式法交叉配血试验结果的影响

To Study the Effect of Different Specimens on the Crossed Blood Test of Card Method

目的分析研究不同标本对卡式法交叉配血试验结果的影响。方法选取我院需要输血治疗的40例做为此次研究对象,分别采集其抗凝血与促凝血与20例抗凝不完全标本进行交叉配血试验,对比不同标本对卡式法交叉配血试验结果的影响作用。结果抗凝血标本检测结果显示,17份标本检出红细胞位于凝胶上方,在这17份标本微管中均能够挑出纤维蛋白凝块,而未经抗凝处理40例血液标本中,红细胞均沉积在微柱凝胶管底部,检测结果显示皆为阴性,抗凝血检测异常率为42.5%,组间数据比较差异明显(χ~2=53.968,P=0.000),有统计学意义;结论不同的标本以及处理方式不当均会导致对卡式法交叉配血试验结果造成影响,未抗凝血液标本纤维蛋白无法分析,不利于快速配血,对于急诊急需输血的患者会造成严重后果,在配血试验中应当洗涤血样红细胞,以提高配血准确性。

Objective To study the effect of different specimens on the crossed blood test of card method. Methods Forty patients with blood transfusion were selected as the subjects. The anticoagulation and coagulation were collected and 20 cases of anticoagulation incomplete specimens were used for cross blood test. The effects of different specimens on the crossover The effect of blood test results. Results The results of anticoagulant test showed that 17 samples were found in red blood cells above the gel, in these 17 specimens of microtubules were able to pick out fibrin clot, and without anticoagulation treatment of 40 patients with blood samples, the red blood cells were deposited in the bottom of the microcolumn gel tube, the test results showed negative, anticoagulant detection abnormal rate was 42.5%, the difference between the two groups significantly(χ~2=53.968, P=0.000), statistically significant. Conclusion Different specimens and improper handling will lead to the cross-matching test results of the card method, in the blood test should be washed blood samples of red blood cells to improve the accuracy of blood distribution.