活性乳清蛋白对乳腺癌荷瘤小鼠化疗期间营养状态的影响

Effects of Active Whey Protein on Nutritional and Immune Status of Mice Bearing Triple-negative Breast Cancer with Chemotherapy

[目的]探究活性乳清蛋白对化疗期间荷瘤小鼠的营养状态、免疫调节和疗效的影响。[方法 ]建立三阴乳腺癌小鼠皮下移植瘤模型,根据干预措施不同随机分为4组,分别为空白对照组(Control组)、紫杉醇化疗组(Control+紫杉醇组)、添加酪蛋白的紫杉醇化疗组(酪蛋白+紫杉醇组)、添加活性乳清蛋白的紫杉醇化疗组(活性乳清蛋白+紫杉醇组)。观察和测量小鼠体重、肿瘤体积、肿瘤重量的变化情况,比较各组小鼠的生存期,检测各组小鼠血液及肿瘤组织中谷胱甘肽(glutathione,GSH)含量。[结果]活性乳清蛋白+紫杉醇组瘤鼠体重下降速度明显缓于酪蛋白+紫杉醇组和Control+紫杉醇组,试验进行第31d活性乳清蛋白+紫杉醇组瘤鼠体重[(20.52±1.10)g],显著高于酪蛋白+紫杉醇组[(19.03±1.76)g]和Control+紫杉醇组[(18.71±0.86)g],差异具有统计学意义。活性乳清蛋白+紫杉醇组瘤鼠肿瘤体积变化与其他各组差异不具有统计学意义。活性乳清蛋白+紫杉醇组生存期较Control+紫杉醇组与酪蛋白+紫杉醇组生存期明显延长。活性乳清蛋白+紫杉醇组肿瘤组织中GSH含量[(34.5±18.0)μmol/L]低于酪蛋白+紫杉醇组[(55.3±23.8)μmol/L]和Control+紫杉醇组[(54.9±11.7)μmol/L]。而血液中,活性乳清蛋白+紫杉醇组血液的GSH含量[(19.1±0.7)μmol/L]高于酪蛋白+紫杉醇组[(13.0±8.8)μmol/L]和Control+紫杉醇组[(15.2±9.7)μmol/L]。但无论是肿瘤组织中还是血液中,各处理组间GSH含量差异均无统计学意义(P>0.05)。[结论]活性乳清蛋白联合化疗减缓小鼠体重下降,改善营养状态,抑制肿瘤增长,延长荷瘤小鼠生存期,改善预后。

[Purpose]To investigate the effect of active whey protein(ABD)on nutritional and immune status of mice bearing triple-negative breast cancer(TNBC)undergoing chemotherapy.[Methods]The triple-negative breast cancer 4T1 cells were inoculated in BALB/c mice.The tumor bearing mice were randomly divided into 4 groups with 6 mice in each group:control group,paclitaxel group,casein+paclitaxel group and ABD+paclitaxel group.The weight of mice,volume of tumor were observed and measured.The overall survival of mice and glutathione(GSH)levels in blood and tumors were analyzed.[Results]Weight of mice in ABD+paclitaxel group[(20.52±1.10)g]was significantly higher than those of casein+paclitaxel group[(19.03±1.76)g]and paclitaxel group[(18.71±0.86)g](P<0.05)on d31.The tumor sizes in ABD+paclitaxel group was lower than that in other groups(P<0.05).The overall survival of the ABD+paclitaxel group was significantly longer than paclitaxel group and casein+paclitaxel group.The GSH levels in tumor tissue of ABD+paclitaxel group,casein+paclitaxel and paclitaxel group were(34.5±18.0)μmol/L,(55.3±23.8)μmol/L and(54.9±11.7)μmol/L,respectively.However,GSH levels in blood of three groups were(19.1±0.7)μmol/L,(13.0±8.8)μmol/L and(15.2±9.7)μmol/L,respectively.There was no significant difference in GSH contents both in blood and tumor tissue among three groups(P>0.05).[Conclusion]Whey protein combined with chemotherapy can attenuate weight loss,improve nutritional status,inhibit tumor growth and prolong survival time in mice bearing triple negative breast cancer.