Marjolin's溃疡鳞状细胞癌组织中K19、β1整合素的表达

Expression of K19 and integrin beta1 in the tissue of squamous cell carcinoma in Marjolin's ulcer

目的检测Marjolin's溃疡鳞状细胞癌中K19、β1整合素的表达情况,探讨Marjolin's溃疡鳞状细胞癌发生与皮肤干细胞的关系。方法选取10例Marjolin's溃疡鳞状细胞癌组织标本、10例皮肤慢性溃疡伴假上皮瘤样增生组织标本、10例原发性皮肤鳞状细胞癌标本、5例正常皮肤组织标本,采用免疫组织化学染色分析皮肤干细胞标记物K19、β1整合素的表达。结果 Marjolin's溃疡鳞状细胞癌组织中,部分癌细胞K19、β1整合素阳性表达,K19为胞浆染色阳性,β1整合素为胞浆、胞膜染色阳性;皮肤慢性溃疡伴假上皮瘤样增生组织在不典型增生部位部分细胞有K19、β1整合素阳性表达;原发性皮肤鳞状细胞癌组织中无K19、β1整合素的表达;K19、β1整合素在正常皮肤毛囊的隆突部位以及基底层仅有少量细胞呈阳性表达。结论皮肤慢性溃疡可以癌变成Marjolin's溃疡鳞状细胞癌,癌变的种子细胞可能来源于溃疡局部的皮肤干细胞。

Objective To explore the relationship between the pathogenesis of squamous cell carcinoma( SCC) in Marjolin's ulcer and the cutaneous stem cells. Methods Ten tissue specimens of SCC in Marjolin' s ulcers,10 tissue specimens of chronic skin ulcers with pseudoepitheliomatous hyperplasia,10 tissue specimens of primary SCC and 10 tissue specimens of normal skin were collected. The K19 and integrin beta1, two typical markers of cutaneous stem cells were studied with the method of immunohistochemistry. Results No K19 and integrin beta1 expressed in primary SCC tissues while K19 and integrin beta1 showed in some tumor cells in the tissues of SCC in Marjolin' s ulcer. The integrin beta1 mainly expressed in the cytoplasmic and cell membrane regions while K19 mainly expressed in cytoplasmic regions. In addition,over expression of K19 and integrin beta1 were seen in some atypia cells of epithelial foot sites in the chronic skin ulcer companied with pseudoepitheliomatous hyperplasia. K19 and integrin beta1 expressed in the regions of the basal layer and the bulge of follicles in normal skin.Conclusion Chronic skin ulcer can turn into SCC and the initial tumor cells may derive from the cutaneous stem cells of the ulcers.