活化诱导胞嘧啶核苷脱氨酶基因多态性与成人哮喘及IgE的相关性研究

Correlation between activation-induced cytidine deaminase gene polymorphism and atopic asthma and plasma IgE in adult

目的:探讨活化诱导胞嘧啶核苷脱氨酶(Activation-induced ey-tidine deaminase.AICDA)8408C/T基因的多态性与成人哮喘及血浆总IgE的关系。方法:采用聚合酶链反应-限制性片段长度多态性分析技术(PCR-RFLP)检测AICDA基因多态性。结果:成人哮喘患者AICDA 8408位T/T基因型频率与对照组相比较差异有显著性(P<0.05),T等位基因频率与对照组相比较差异无显著性(P>0.05)。成人哮喘组T/T基因型血浆总IgE高于C/C、C/T基因型(P<0.05)。结论:成人哮喘患者AICDA 8408位T/T基因型与成人哮喘的发病及高血浆总IgE均相关。

AIM: To explore whether the activation-induced cytidine deam-inase(AICDA) gene 8408 C/T polymorphism is related to adult atopic asthma and the level of plasma IgE. METHODS: The polymorphism of AICDA gene was detected by PCR and restriction fragment length polymorphism ( PCR-RFLP). RESULTS: The frequencies of 8408T/T genotype had significant difference (P<0.05) between adult asthma patients and control group, while the frequencies of T allele between two groups were not significantly different. The total plasma IgE level in adult atopic asthma patients with 8408T/T genotype was higher than that in the patients with C/C and C/T genotypes (P < 0. 05). CONCLUSION: The 8408 T/T genotype of AICDA is correlated with atopic asthma and total plasma IgE level in adult.