一氧化氮在巨噬细胞的细胞毒效应中的作用

The role of nitric oxide in the cytotoxic effect of macrophages

目的:探讨一氧化氮(NO)对巨噬细胞细胞毒效应的影响。方法:将20只昆明种小鼠分为两组,一组皮下接种S180横纹肌肉瘤细胞,另一组作为正常对照组。分别取两组小鼠腹腔灌洗液中的巨噬细胞与K562肿瘤细胞共同培养,检测在培养液中加入左旋精氨酸(L-Arg)和G-单甲基左旋精氨酸(L-NAME)后巨噬细胞杀伤率的改变。结果:正常小鼠腹腔灌洗液中的巨噬细胞在L-Arg和L-NAME存在的情况下细胞毒效应没有改变;荷瘤小鼠腹腔灌洗液中的巨噬细胞在L-Arg组和空白组中的细胞毒效应明显增强。结论:NO是巨噬细胞细胞毒作用的一个效应分子。

AIM: To explore the role of nitric oxide (NO) in cytotoxicity of macrophages. METHODS: 20 mice of Kunming species were divided into two groups. One group were injected sub-cutaneously with rhabdosarcoma cells S180 and the other were normal controls. The macrophages in peritoneal lav-age fluid of two groups of mice were co-cultured with tumor cells K562. L-arginine(L-Arg) or NG-nitro-L-arginine methyl este(L-NAME) was added to the culture medium, and the cytotoxicity of macrophages was measured. RESULTS: Macrophages from tumor-bearing mice had stronger cytotoxicity than those from nomal mice. L-Arg increased and L-NAME decreased the cytotoxicity of macrophages from tumor-bearing mice, but both had no effect on the cytotoxicity of macrophages from nomal mice. CONCLUSION: NO is an effector molecule in cytotoxicity of activated macrophages.