内毒素体外诱导巨噬细胞炎症蛋白-2γ的表达

Stimulating the expression of macrophage inflammatory protein-2γ by lipopolysac- charide in vitro

目的:体外研究内毒素对巨噬细胞炎症蛋白-2γ(MIP-2γ)表达的作用。方法:选用小鼠单核-巨噬细胞株RAW264.7及原代培养的BALB/c小鼠肾脏细胞用LPS刺激,以实时荧光定量RT-PCR检测不同时间点MIP-2γmRNA表达水平的变化。结果:RAW264.7细胞在LPS刺激后2 h,MIP-2γ.mRNA表达水平快速到达峰值(较正常水平增加约7倍),以后缓慢下降,至刺激后16 h基本恢复正常水平。原代培养肾脏细胞经LPS刺激后12 h,MIP-2γmRNA表达水平增长约50倍。结论:LPS在体外可明显诱导单核-巨噬细胞和肾脏细胞MIP-2γ mRNA的表达,提示MIP-2γ参与了炎症过程。

AIM: To study the expression level of macrophage inflammatory protein-2γ(MIP-2γ) stimulated by lipopolysaccharide (LPS)in vitro. METHODS: Murine monocyte-macrophage-like cell line RAW264.7 and BALB/c mice primary kidney cell culture were stimulated with LPS. MIP-2γ mRNA expression level in these cells was detected by real-time fluorescence quantitative RT-PCR. RESULTS: MIP-2γ mRNA expression level of RAW264. 7 cell line increased quickly and reached the peak (7 times the normal level) within 2 hours after LPS stimulation and dropped to the base level within 16 hours, while that of cultured promary kidney cells of BALB/c mice elevated about 50 times compared with base level 12 hours following LPS stimulation. CONCLUSION: LPS can significantly stimulate the expression of MIP-2γ mRNA, suggesting that MIP-2γ may play some role in inflammatory process.