木枣叶片再生植株及其变异系过氧化物酶同工酶分析

Analysis of peroxidase isoenzyme of the reproductive plants and the variable system of Ziziphus jujuba Mill var.muzao leaves

用聚丙烯酰胺凝胶电泳分析了木枣叶片再生植株及其5种变异系过氧化物酶 POD 同工酶.结果表明:木枣叶片再生植株过氧化物酶同工酶由3个基因位点编码,位点1编码的是杂合三聚体酶,位点2和位点3编码的是纯合二聚体酶,其变异系中有不能表达位点2和位点3的植株,变异较大.也有完整表达3个基因位点的植株,且酶活性高.

The peroxidase isoenzymes of reproductive plants and five variable system of Z.jujuba\% Mill var.\%muzao\% leaves were studied using polyacrylamide gel eletrophoresis.The result showed that peroxidase isoenzymes of reproductive plants of Z.jujuba\% Mill var.\%muzao\% leaves are coded by three genetic locus (L),L1 codes heterozygous trimeric enzyme,L2 and L3 code homozygous dimeric enzyme,some of plants of variable system didnt express L2 and L3,having shown much variation.Some express three locuses completely.Their activity are strong.