摘要
目的 :探讨建立基底动脉栓塞动物模型的方法。方法 :结扎实验豚鼠双侧颈动脉 ,运用4 0 %三氯化铁 (FeCl3)溶液诱导基底动脉栓塞 ,采用激光多普勒流量仪测量诱导栓塞前后耳蜗血流量 (CBF)的变化 ,以压力传感器和前置放大器等装置同步监测平均动脉压 (MABP)的变化。结果 :4 0 %FeCl3溶液诱导 5～ 10min ,CBF开始下降 ,随着诱导时间的延长 ,CBF下降进一步加大 ,诱导 5 0min时 ,CBF下降达 (6 4.5± 4 .2 ) % ,与诱导前比较 ,差异有极显著性意义 (P <0 .0 1)。整个实验期 ,CBF的均值为 (81.3± 12 .0 ) % ,与诱导前比较 ,差异亦有极显著性意义 (P <0 .0 1)。MABP无明显变化。结论 :运用 4 0 %FeCl3溶液能诱导豚鼠基底动脉栓塞 。
Objective:The present work was undertaken to establish an animal model of basilar arterial thrombosis.Method:The bilateral carotid arterial of guinea pigs were ligated and 40%ferric chloride solution was applied to basilar artery topically. The blood flow of cochlea(CBF) was measured continuously with laser doppler flow meter. The mean arterial pressure (MABP) was recorded simultaneously. The data was analysed by t test.Result:After the administration of 40% FeCl 3 to basilar artery, The CBF began decrease significantly from 5 to 10 min, and declined more great along with the prolongation of FeCl 3 effect. The CBF reached to ( 64.5 ± 4.2 )% of the base level at 50 min after FeCl 3 exposure. A total average decrease of CBF was ( 81.3 ± 12.0 )% of the base level throughout the experiment period. The MABP had no significant change.Conclusion:The topical application of 40% FeCl 3 solution can offer an ideal model of basilar artery thrombosis
出处
《临床耳鼻咽喉科杂志》
CAS
CSCD
北大核心
2003年第6期364-366,共3页
Journal of Clinical Otorhinolaryngology
基金
卫生部优秀科技人才专项基金资助项目(No.1995144)
教育部留学回国人员科技基金资助项目(No.1996644)
湖南省教育厅基金资助项目 (No .0 1C179)
关键词
基底动脉栓塞
疾病模型
动物
三氯化铁
耳蜗血流量
Basilar arterial thrombosis
Disease models, animal
FeCl 3
Blood flow of cochlea
