大鼠皮肤切创愈合过程中ICAM-1及P选择素的表达

The expression of ICAM-1 and P-selectin in the healing process of incised wounds of rats

目的 探讨细胞间粘附分子 1(ICAM 1)及P选择素的表达变化与损伤时间的关系。方法 应用免疫组织化学技术 ,对实验大鼠不同损伤时间的生前伤 ( 5min～ 7d)及死后伤 ( 5～ 3 0min)皮肤组织中ICAM 1及P选择素的表达变化进行观察。结果 在生前损伤组中 ,ICAM 1最早在伤后 1h ,最迟至伤后 3d在表皮层中呈阳性表达 ;P选择素最早在伤后 10min ,最迟至伤后 5h即在血管内皮细胞中呈阳性表达。此外 ,ICAM 1还在炎症细胞及纤维母细胞中表达 ,且随损伤时间变化而呈规律性变化。在时间段分组 ,ICAM 1在第Ⅰ组 ( 5min～ 1h)中阳性细胞率极低( 0 41± 0 73 % ) ,第Ⅱ组 ( 3h～ 7h)及第Ⅲ组 ( 9h～ 12h)均呈显著性增高 ( 9 79± 3 74% ,2 3 3 3± 1 10 % ) ,至第Ⅳ组 ( 1d)达高峰 ( 3 0 5 8± 2 65 % ) ,其后逐渐减少。ICAM 1及P选择素在死后伤中均呈阴性表达。结论 ICAM 1及P选择素可作为法医学损伤时间判定的有效指标。

Objective Explore the expression of intercellular adhesion molecule-1(ICAM-1) and Pselectin in the healing process for possible application in timing of incised wounds. Method An immunohistochemical study on the expression of ICAM-1 and P-selectin was performed on rats vital skin incised wounds (5min～7d)using postmortem incisions(5～30min)as control.Results In the vital skin incisions,positive stain of ICAM-1 was observed on epidermis began from 1h and lasted till 3d, while the P-selectin stain was observed on vascular endothelium as early as 10min after incision and lasted 5h; in addition, the expression of ICAM-1 was also found in the inflammatory cells and fibroblasts,the lasting period varied from group to group (groupⅠ 5min～1h,groupⅡ 3～7h,group Ⅲ 9～12h,groupⅣ 1d,groupⅤ 3d,groupⅥ 5～7d.).The positive ratios of ICAM-1 among the cells were very low in group Ⅰ(0.41±0.73%),and increased considerably in group Ⅱ(9.79±3.74%) and group Ⅲ(23.33±1.10%),maximized in group Ⅳ(30.58±2.65%),then decreased in group Ⅴ and Ⅵ.There were no changes of ICAM-1 and P-selectin in the postmortem incision group. Conclusion ICAM-1 and P-selectin are considered potentially useful markers for wound age determination in forensic practice.