原发性肝癌TACE治疗前后血清VEGF含量的变化

The changes of serum vascular endothelial growth factor before and after transcatheter arterial chemo-embolization in patients with hepatocellular carcinoma

目的:研究肝细胞癌(HCC)患者经肝动脉灌注化疗栓塞术(TACE)后外周血血清中血管内皮生长因子(VEGF)的表达及其相关因素的探讨。方法:运用酶联免疫吸附测定法(ELISA)定量检测28例HCC患者TACE术前、术后即刻,术后1天、3天、5天、7天、14天、28天的血清VEGF的含量,并在术前及术后1天测定肝功和外周血血小板数量。结果:TACE术后1天血清VEGF升高达峰值,之后逐步下降,术后14天又开始升高。术后1天肝功(ALT和AST)升高达峰值,以后下降。术后1天较术前血清VEGF的变化与相应肝功变化,外周血血小板数量变化及术中碘化油用量无相关性。结论:TACE术后外周血VEGF含量的变化,反映了HCC患者TACE术后肿瘤组织缺氧及肿瘤血管生成状况,依据VEGF分泌规律,TACE术后选择合适时机进行抗肿瘤血管生成治疗,有望提高TACE的疗效。

Objective: To investigate the levels of serum vascular endothelial growth factor (VEGF) and other relevant factors of patients with hepatocellular carcinoma(HCC) who underwent transcatheter arterial chemo-embolization(TACE). Methods: Twenty-eight patients with HCC were used for study. Serum were isolated from peripheral blood before and 0, 1, 3, 5, 7, 14 and 28 days after TACE, and also measured hepatic function (ALT and AST) and peripheral platelet before and 1 day after TACE. VEGF were measured by enzyme-linked immunosorbent assay using a specific anti-human VEGF antibody. Results: Serum VEGF levels decreased after TACE, then elevated and reached a peak at 1 day after TACE, then gradually decreased, rised again at 14 days after TACE. There is no relevant between the changes of serum VEGF levels of 1 day after and before TACE and changes of hepatic function, peripheral platelet count and the quantity of the Iodized oil during TACE. Conclusion: The changes of serum VEGF reflected hypoxia and angiogenesis of tumor tissue in patients with HCC, if we choose the correct time for anti-angiogenesis after TACE, the therapeutic effect would be enhanced.