[Ru(bpy)_2dppz]^(2+)的合成及其与DNA的相互作用研究

Synthesis of [Ru(bpy)_2dppz]^(2+) and its Interaction with DNA

首先通过两步反应合成了"光开关"分子,[Ru(bpy)2dppz]2+(简称Ru-dppz; bpy=2,2'-联吡啶,dppz=二吡啶[3, 2-a; 2', 3'-c]吩嗪),并分别用紫外-可见吸收光谱法、核磁共振和质谱表征其结构。再利用荧光法考察了Ru-dppz与双链DNA(dsDNA)的相互作用。结果表明:随着Ru-dppz与dsDNA的结合,Ru-dppz荧光强度增加。根据荧光滴定实验结果,计算所得Ru-dppz与dsDNA的结合常数为1.5×107L/mol,结合比为0.14。

The well-known 'light switch' molecule, [Ru(bpy)2 dppz]2+(denoted as Ru-dppz), was synthesized in two steps. The structure of Ru-dppz was characterized with UV-visible spectrophotometry, nuclear magnetic resonance(NMR), mass spectrometry(MS), respectively. Next, the fluorescence properties of Ru-dppz with double stranded DNA(dsDNA) were investigated in detail. We found that the fluorescence intensity of Ru-dppz increased as the addition of DNA. It concluded that Ru-dppz can serve as a true molecular 'light switch' for dsDNA. The fluorescence titrate experiments showed their binding constant and binding-site size with dsDNA were 1.5×107 L/mol and 0.14(dye to base pairs ratio, dbpr), respectively,which agreed reasonably well with those reported in the literatures.