摘要
目的:研究金莲花中荭草苷和牡荆苷的抗氧化作用。方法:采用D-半乳糖腹腔注射(ip)致亚急性衰老小鼠模型,以维生素E(20 mg·kg-1)为阳性对照,荭草苷和牡荆苷各以40,20,10 mg·kg-1剂量连续灌胃(ig)给药8周后,对衰老小鼠血清总抗氧化能力和脑、肝、肾组织抗氧化酶系活性、细胞膜转运离子ATP酶活性、丙二醛含量进行测定。结果:荭草苷和牡荆苷均能提高小鼠血清总抗氧化能力且能提高组织中超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶及Na+-K+-ATP酶、Ca2+-Mg2+-ATP酶的活性,并具有降低组织中丙二醛含量的作用,40 mg·kg-1的荭草苷和牡荆苷与维生素E抗氧化作用相当。结论:荭草苷和牡荆苷具有一定的抗氧化能力,可能是其抗衰老的作用机制之一。
Objective:To study the antioxidant effects of orientin and vitexin from Trollius chinensis Bunge. Methods:Daily intraperitoneal injection of D-galactose for 8 weeks was used to establish experimental aging in mice. Different groups of mice were treated with 40,20 or 10 mg·kg-1 orientin,vitexin,or a positive control(vitamin E)via intragastric administration for an additional 8 weeks. The total antioxidant capacity in the serum,activities of the antioxidant enzyme and the cell membrane transport ion ATP enzyme,and the level of malondialdehyde in the liver,brain and kidneys were determined. Results:Orientin,vitexin,and vitamin E produced a significant increase in total antioxidant capacity,in the serum,and the levels of superoxide dismutase,catalase and glutathione peroxidase,Na+-K+-ATP enzyme,and Ca2+-Mg2+-ATP enzyme in the liver,brain and kidneys. In addition,they significantly reduced the malondialdehyde levels in the liver,brain and kidney. The 40 mg·kg-1 dose of orientin and vitexin had similar antioxidant effects as vitamin E. Conclusion:Orientin and vitexin have anti-aging effects possibly through their antioxidant activities.
出处
《神经药理学报》
2012年第3期1-6,共6页
Acta Neuropharmacologica
基金
张家口市科学技术研究与发展指令计划项目(No.11110015D)
河北北方学院自然基金(2011)
