荭草苷锌配合物的合成、表征及其抗氧化活性研究

Studies on Synthesis and Structure Characterization and Biological Activity of Orientin-Zinc Complexes

目的:优化荭草苷锌配合物合成工艺,对荭草苷和荭草苷锌配合物进行体外抗氧化活性比较研究。方法:运用荭草苷和乙酸锌合成荭草苷锌配合物,采用元素分析法、等离子发射光谱法、紫外光谱法、红外光谱法、热重分析法、核磁共振法研究荭草苷锌配合物的结构表征。选择羟基自由基(·OH),1,1-二苯基-2-三硝基苯肼自由基(·DPPH)产生体系为实验体系,考察荭草苷和荭草苷锌配合物的抗氧化活性。结果:荭草苷与乙酸锌配位反应形成配合物的最佳工艺为:以无水乙醇为溶剂、反应p H 8.5,反应温度65℃,搅拌时间8 h。得到荭草苷锌配合物可能的化学分子式[Zn3(C21H15O11)2]·4H2O和化学结构式。荭草苷和荭草苷锌配合物都具有清除·OH、·DPPH的能力,且呈一定的量效关系。在1.0~8.0μg·m L-1范围内,荭草苷锌配合物各剂量组在清除·OH、·DPPH能力上高于荭草苷同等剂量组。结论:荭草苷锌配合物在抗氧化活性上优于荭草苷配体。

Objective:To optimize the synthetic process conditions of and study the antioxidation activity of orientin and orientin zinc complexes in vitro,Methods:Using elemental analyzer,plasma emission spectrometer,ultraviolet spectrometer,infrared spectrometer,thermogravimetric analyzer and nuclear magnetic resonance instrument,the structural characterization of orientin zinc complexes was observed,taking superoxide anion radicals,hydroxyl radicals,1,1-dipheny1-2-picrylhydrazyl radical 2,2-dipheny1-1-(2,4,6-trinitrophenyl) hydrazyl(DPPH) radical generation system as experimental system,to examine the antioxidant activity of orientin and orientin-zinc complexes.Results:The best coordination reaction optimum process of complexes formed by orientin and zinc acetate was shown as follows.Taking anhydrous ethanol as solvent,reaction pH was 8,reaction temperature was65℃,and reaction time was 8 h.The orientin-zinc complexes may be the chemical formula[Zn_3(C_(21)H_(15)O_(11))_2]·4H_2O and chemical structural formula.Orientin and orientin-zinc complexes have clear ability of removing ·OH and ·DPPH,and the effect of orientin-zinc complexes in each dose group was more obvious than equivalent dose orientin at 1.0~8.0μg·mL^(-1).Conclusion:Orientin-zinc complexes is more superior than orientin on the activity of antioxidation.