响应面法优化大孔树脂纯化百合多糖研究

Optimization Macroporous Resin Purification Process of Lilium Brownii Polysaccharide with Response Surface Analysis

以纯化产率和多糖含量为评价指标,采用响应面法对D315大孔树脂纯化百合多糖纯化工艺进行优化。研究了层析柱流速、粗多糖浓度、上柱温度和粗糖溶液p H值四个因素对百合多糖纯化产率和产品中多糖含量的影响。结果表明D315大孔树脂纯化百合多糖最佳工艺为样品溶液流速3m L/min,粗多糖浓度3.6mg/m L,上柱温度46℃,粗糖溶液p H值为7。在此工艺条件下产品中多糖纯度可达89.62%,纯化产率为75.57%。在最优工艺条件下,50g D315大孔树脂一次性处理量为浓度为3.6mg/m L的粗多糖提取液500~600m L,纯化效果最好。

The purification process of Lilium Brownii polysaccharide with D315 resin was optimized by the response surface analysis method based on the purification yield and the content of polysaccharide as the evaluation index. The effects of flow rate of the column, the concentration of raw sugar, the influence of column temperature and raw sugar solution p H on the purification yield and the content of polysaccharide were discussed. The results showed the best optimum purification process is that the flow rate of the columns is 3mL/min, the concentration of raw sugar is 3.6mg/m L, the column temperature is 46 ℃ and the raw sugar solution pH is 7. Under the optimum purification condition, the average of content and the purification yield of polysaccharide is 89.62% and 75.57%, respectively. The average amount of the polysaccharide extraction liquid with concentration of 3.6mg/mL is 500~600 mL for 50gD315 under the optimization conditions.