摘要
羟钴胺属于维生素B12的基本成员,因其分子结构中含有Co—OH基团而使得羟钴胺呈弱碱性;弱碱化合物易与色谱柱中的硅胶产生氢键键合作用,因而使色谱峰拖尾,色谱峰的拖尾在定量分析中严重影响了结果的计算。笔者通过色谱条件的优化有效解决了羟钴胺在色谱分析中的拖尾现象,有效解决了羟钴胺的含量及杂质含量计算不准的问题,为精准用药提供了科学依据。
Hydroxycobalamin belongs to VB12 series, which presents alkalescence as Co—OH group is obtained in the molecular structure; Alkaline compounds is easy to produce Hydrogen Bonding effect with the silica gel in chromatographic column, therefore the chromatographic peak is trailing, the trailing impact the result calculation seriously in the quantitative analysis. The writer solved the trailing phenomenon effectively in the chromatographic analysis through optimizing chromatographic condition, also solve the calculation problem for Hydroxycobalamin assay and impurity, and provide the scientific evidence for accurate medication.
