摘要
目的开发制备用于体外培养小鼠胰岛细胞团用的鼠尾胶。方法剥离大鼠鼠尾制作鼠尾胶原,并用该胶原在不同条件下培养小鼠胰岛细胞团,用FD-PI染色观察胰岛活性。结果用自制鼠尾胶原培养胰岛细胞团24 h后,细胞团依然圆整、活性好。结论用本法制备的鼠尾胶可用于小鼠胰岛细胞团的培养,胰岛细胞团在该鼠尾胶中可保持活性。
Objective:Exploit and make the rattail collagen used for culturing the mice islet cells in vitro.Process: Stripping the rat's tail to make rattail collagen,and use this collagen to culture mice islet cells under the different conditions, with FD-PI staining to observe the islet activity.Result: After 24 hours, the cultivation of islet cells with the self-control rattail collagen, the cell mass is still round and has good activity.Conclusion: The rattail collagen made with this method can be used into the cultivation of mice islet cell mass, the islet cell mass can keep activity in this rattail collagen.
基金
温州医科大学本专科学生科研课题(wyx201401065)
宁波市科技计划项目(2013A610270)
