大豆异黄酮对动脉粥样硬化大鼠炎症分子相关基因表达的影响

Effect of soy isoflavones on the expression of inflammatory molecules gene in atherosclerosis rats

目的:研究大豆异黄酮(soybean isoflavone,SIF)对动脉粥样硬化(atherosclerosis,As)大鼠血管中促As炎症相关基因表达的影响。方法:建立As大鼠模型,随机分成正常组、模型组、模型给药组和正常给药组;HE染色观察各组大鼠胸主动脉病理改变;实时PCR和免疫组化检测血管中促As炎症的相关基因表达量。结果:模型组胸主动脉严重萎缩、钙化,出现硬化斑块;模型给药组As病变较模型组明显减轻;正常组和正常给药组胸主动脉无病理改变。模型组胸主动脉中单核细胞趋化蛋白-1(monocyte chemotactic protein-1,MCP-1)、CC类趋化因子受体2(C-C chemokine receptor,CCR2)、单核细胞趋化蛋白-1诱导蛋白(MCPIP)和C反应蛋白(CRP)mRNA表达量较正常组明显升高(P<0.01或P<0.05);模型给药组上述4种基因mRNA表达量均较模型组明显降低(P<0.01或P<0.05);正常组与正常给药组4种基因mRNA表达量间的差异无统计学意义。免疫组化结果显示,与正常组比较,模型组MCPIP、MCP-1、核因子κB(NF-κB)阳性染色和范围均增多(P<0.01),模型给药组3种蛋白的阳性染色和范围都较模型组明显减少(P<0.05);正常组和正常给药组3种蛋白表达间的差异无统计学意义。结论:大豆异黄酮能显著降低促As炎症的相关基因表达,明显抑制大鼠As病变。

Objective: To investigate the effects of soy isoflavones(SIF) on the expression of gene for proinflammatory of atherosclerosis(As) in the As rats. Methods: To establish the rats model of As,were randomly divided into normal control,model,model + SIF and normal + SIF group; the pathological changes of thoracic aorta of rats in each group were observed by HE staining; Real time-PCR and immunohistochemical detection of the expression of gene for proinflammatory of As in vessels. Results: The model group showed severe atrophy and calcification of thoracic aorta compared with normal control group,even appear atherosclerotic plaques; compared with model group,model + SIF group showed actions in lessening the atherosclerosis lesion obviously; both normal control group and normal + SIF group without pathological changes of thoracic aorta. Compared with model group,mRNA level of MCP-1 / CCR2,MCPIP and CRP were higher than that in normal control group of thoracic aorta(P < 0. 01 or P < 0. 05); mRNA level of the four genes in model + SIF group of thoracic aorta were lower than that in model group; there was not significant difference in mRNA level of the four genes between normal control group and normal + SIF group. Immunohistochemistry results showed that expression of positive staining and range of MCPIP,MCP-1,and NF-κB in model group were higher than in normal control group(P <0. 01),the expression of positive staining and range of the three protein in model + SIF group were lower than in model group( P < 0. 05). Between the normal control group and the normal + SIF group,the expression of positive staining and range of the three protein were no significant difference. Conclusion: SIF can obviously decrease the expression of genes related to proinflammatory of As,and inhibit the atherosclerosis formation in rats.