NDRG1、P21及VEGF在肺腺癌组织中的表达及临床意义

Expression of NDRG1,P21 and VEGF in lung adenocarcinoma tissue and its clinical significance

目的:观察N-myc下游调节基因1(N-myc downstream regulated gene1,NDRG1)、P21及血管内皮生长因子(vascular endothelial growth factor,VEGF)基因在肺腺癌中的表达及在肺腺癌发生、发展中的作用及意义。方法:应用免疫组织化学法检测20例正常肺组织和94例肺腺癌组织中NDRG1、P21及VEGF蛋白的表达情况,并分析三者表达与临床病理参数及患者预后的相关性。结果:在正常肺组织及肺腺癌组织中,NDRG1阳性率分别为90.0%(18/20)和52.1%(49/94),P21阳性率分别为80.0%(16/20)和33.0%(31/94),VEGF阳性率分别为20.0%(4/20)和53.2%(50/94),3种蛋白阳性率比较差异均具有统计学意义(P均<0.05)。肺腺癌组织中NDRG1、P21及VEGF的表达与肺腺癌患者年龄、性别、组织分化程度及TNM分期均无相关性(P均>0.05),而与淋巴结转移明显相关(P<0.05)。Kaplan-Meier生存分析显示,NDRG1、P21和VEGF表达与生存明显相关(P均<0.01)。结论:肺腺癌组织中NDRG1和P21表达下调,而VEGF表达上调,联合检测三者的表达可能对判断预后具有重要价值。

Objective:To observe the expression of N-myc downstream regulated gene1 (NDRG1),P21 and vascular endothelial growth factor(VEGF) in lung adenocarcinoma tissue,and to elucidate the mechanism of lung adenocarcinoma invasion and metastasis.Methods:The expressions of NDRG1,P21 and VEGF were detected by immunohistochemistry in 20 cases of normal lung tissue and in 94 cases of lung adenocarcinoma tissue.The correlation among NDRG1,P21 and VEGF expressions in lung adenocarcinoma tissue was investigated by Spearman rank correlation analysis.The relationship between NDRG1 expression and clinicopathological characteristics was investigated by Kaplan-Meier survival curve analysis.Results:In normal lung tissue and lung adenocarcinoma tissue,the positive immunoreactivity rate of NDRG1 was 90.0％ (18/20) and 52.1％ (49/94),respectively ; P21 was 80.0％ (16/20) and 33.0 ％ (31/94),respectively; VEGF was 20.0％ (4/20) and 53.2％ (50/94),respectively.The expressions of NDRG1 and P21 was negatively correlated with lymphatic metastasis,while the expression of VEGF was positively correlated with lymphatic metastasis.Univariate survival analysis indicated that the expressions of NDRG1,P21 and VEGF were associated with patient survival.Conclusion:The expressions of NDRG1 and P21 in lung adenocarcinoma tissue were down-regulated while VEGF expression was up-regulated.The joint detection of NDRG1,P21 and VEGF might provide some guiding significance to judge the prognosis of lung adenocarcinoma.