ZIC1在结直肠癌中的表达及其对结肠癌细胞增殖能力的影响

ZIC1 expression in colorectal cancer tissue and its effect on colon cancer cell proliferation

目的:研究结直肠癌组织中小脑锌指结构1(zinc finger of the cerebellum 1,ZIC1)基因的表达及其对结肠癌HCT-8细胞增殖能力的影响。方法:构建192例结直肠癌组织芯片,免疫组织化学法检测结直肠癌组织及相应癌旁组织中ZIC1的表达,分析其表达与临床病理参数之间的关系;构建pc DNA3.1-ZIC1表达载体(实验组)和pc DNA3.1空载体组(阴性对照组),转染结肠癌HCT-8细胞,蛋白质印迹和RT-PCR法检测实验组、阴性对照组和空白对照组(常规无转染培养)细胞ZIC1的表达水平,MTT法检测3组细胞增殖能力的变化。结果:结直肠癌组织中ZIC1的阳性表达率(41.1%)明显低于癌旁组织(68.8%,χ2=29.550,P=0.000);ZIC1的表达与Dukes分期、分化程度、浸润深度及淋巴结转移相关(P均<0.05),而与年龄、性别无关(P>0.05)。RT-PCR及蛋白质印迹检测结果显示,实验组细胞ZIC1的表达明显高于阴性对照组及空白对照组;MTT法检测结果显示,实验组HCT-8细胞增殖能力明显下降。结论:ZIC1基因可能参与了结直肠癌的发生、发展。

Objective: To investigate the expression of zinc finger of the cerebellum 1( ZIC1) gene in the colorectal cancer tissue and its impact on the proliferation of colon cancer HCT-8 cells. Methods: Tissue microarray blocks containing 192 colorectal cancer tumor specimens were conducted. Expression of ZIC1 in colorectal cancer tumor specimens and adjacent cancer tissues was analyzed using immunohistochemistry; the relationship between ZIC1 expression and clinical pathological parameters was analysed. The pc DNA3. 1-ZIC1 expression vector( experiment group) and pc DNA3. 1 empty carrier group( negative control group) were constructed to transfect HCT-8 cells,Western blotting and RT-PCR were used to detect ZIC1 expression among the experiment group,negative control group and blank control group( no transfection cultivation). The changes of cells proliferation of three groups were determined by MTT. Results: The positive expression rate of ZIC1 in colorectal cancer tissue was higher than adjacent cancer tissues( 41. 1%vs 68. 8%,P < 0. 05). The ZIC1 expression in colorectal cancer tissues was correlated with Dukes staging,degree of differentiation,infiltration depth and lymph node metastasis( P < 0. 05),whereas incorrelated with age and gender( P > 0. 05). RT-PCR and Western blotting showed the expression of ZIC1 in experiment group was significantly higher than that in negative control group and blank control group; MTT showed the cell proliferation in experiment group reduced markedly. Conclusion: ZIC1 might participate in the development and progression of colorectal cancer.