摘要
目的:建立肠炎相关性结直肠癌的小鼠模型。方法:给予小鼠单次小剂量腹腔注射氧化偶氮甲烷(azoxymethane,AOM)联合2%葡聚糖硫酸钠(dextran sodium sulpate,DSS)自由饮用,建立肠炎相关性结直肠癌小鼠模型。对照组小鼠给予PBS腹腔注射后普通饮水。每天观察症状,2%DSS 5 d,普通饮水16 d,此21 d为1个循环,共进行4个循环,结束后,断颈处死小鼠,取脾脏、肠系膜淋巴结、结肠,观察并比较两组小鼠脾脏,肠系膜淋巴结大体改变和结肠HE染色情况。结果:与对照组比较,实验组小鼠脾脏、肠系膜淋巴结明显增大,结肠远端出现肿瘤。HE染色结果显示,实验组发病小鼠结肠黏膜层及黏膜下层出现广泛的炎症细胞浸润,腺体组织结构紊乱、破坏,出现腺管样结构,具有组织异型性。结论:单剂量AOM与DSS联合使用可在短期内诱导小鼠结肠肿瘤的发生。
Objective: To establish a mice model of colitis associated cancer. Methods: The colitis associated cancer was induced by a single intraperitoneal injection of 12. 5 mg / kg azoxymethane( AOM) and stepped by four cycles oral administration of 2% dextran sodium sulpate( DSS). The occult blood was observed everyday. At the end of the fouth cycle,the mice were killed for observing the spleen,mesenteric lymph nodes,colon; colon tissues were harvested for mucosa morphological changes under light microscopy. Results: Compared to the control group,the spleens,mesenteric lymph nodes were enlarged and the tumorigenesis was found in distal colon in the experiment group; and there were a lot of inflammation cell infiltration and dysplasia in colon. Conclusion: Administration of AOM combined with DSS could induce a mice model of colorectal cancer in a short period.
出处
《江苏大学学报(医学版)》
CAS
2015年第1期19-21,共3页
Journal of Jiangsu University:Medicine Edition
基金
国家自然科学基金资助项目(31170849)
江苏省自然科学基金资助项目(BK2011472)