氧化偶氮甲烷和葡聚糖硫酸钠致肠炎相关性结直肠癌小鼠模型的建立

Establishment of AOM / DSS induced a mice model of colitis associated cancer

目的:建立肠炎相关性结直肠癌的小鼠模型。方法:给予小鼠单次小剂量腹腔注射氧化偶氮甲烷(azoxymethane,AOM)联合2%葡聚糖硫酸钠(dextran sodium sulpate,DSS)自由饮用,建立肠炎相关性结直肠癌小鼠模型。对照组小鼠给予PBS腹腔注射后普通饮水。每天观察症状,2%DSS 5 d,普通饮水16 d,此21 d为1个循环,共进行4个循环,结束后,断颈处死小鼠,取脾脏、肠系膜淋巴结、结肠,观察并比较两组小鼠脾脏,肠系膜淋巴结大体改变和结肠HE染色情况。结果:与对照组比较,实验组小鼠脾脏、肠系膜淋巴结明显增大,结肠远端出现肿瘤。HE染色结果显示,实验组发病小鼠结肠黏膜层及黏膜下层出现广泛的炎症细胞浸润,腺体组织结构紊乱、破坏,出现腺管样结构,具有组织异型性。结论:单剂量AOM与DSS联合使用可在短期内诱导小鼠结肠肿瘤的发生。

Objective: To establish a mice model of colitis associated cancer. Methods: The colitis associated cancer was induced by a single intraperitoneal injection of 12. 5 mg / kg azoxymethane( AOM) and stepped by four cycles oral administration of 2% dextran sodium sulpate( DSS). The occult blood was observed everyday. At the end of the fouth cycle,the mice were killed for observing the spleen,mesenteric lymph nodes,colon; colon tissues were harvested for mucosa morphological changes under light microscopy. Results: Compared to the control group,the spleens,mesenteric lymph nodes were enlarged and the tumorigenesis was found in distal colon in the experiment group; and there were a lot of inflammation cell infiltration and dysplasia in colon. Conclusion: Administration of AOM combined with DSS could induce a mice model of colorectal cancer in a short period.