miR-93在急性髓系白血病中的表达及其与miR-378表达的相关性

Expression of miR-93 in AML and association between miR-93 and miR-378

目的:探讨微小RNA-93(miR-93)在急性髓系白血病(acute myeloid leukemia,AML)中的表达特点及其与miR-378表达的相关性。方法:用实时定量逆转录-聚合酶链反应(RQ-PCR)检测84例AML患者及16例健康对照骨髓单个核细胞中miR-93成熟体的表达水平。分析miR-93高表达对AML患者预后的影响及其与miR-378表达的相关性。结果:84例AML患者中有14例过表达miR-93(16.7%)。miR-93高表达组患者除白细胞数(3.11×109/L)明显低于低表达组(14.55×109/L,P=0.003)外,其他参数如性别、年龄、血红蛋白和血小板等均无明显差异。与其他核型分组[10/38(26.3%)]比较,中等预后核型分组中miR-93高表达的频率明显下降[4/46(8.7%),χ2=4.652,P=0.041]。Spearman相关系数分析显示,miR-93表达率与miR-378表达率呈正相关(r=0.321,P<0.01)。结论:miR-93在AML中表达率明显增加,且与miR-378表达率呈正相关。miR-93联合miR-378的表达可能作为AML诊断的参考依据。

Objective: To investigate the expression of miR-93 in acute myeloid leukemia( AML) patients and the correlation between miR-93 and miR-378. Methods: Real-time quantitative PCR was performed to detect the expression level of miR-93 in AML patients. The clinical significance of miR-93 expression in AML was investigated. Results: miR-93 overexpression was identified in 14( 16. 7%) of 84 AML patients. The patients with miR-93 overexpression had lower white blood cell level than those without miR-93 overexpression( 3. 11 × 109/ L versus 14. 55 × 109/ L,respectively,P = 0. 003). Compared with other karyotype classifications,the frequency of miR-93 overexpression in the intermediate ones was lower [4 /46( 8. 7%) versus 10 /38( 26. 3%),P = 0. 041]. Spearman correlation analysis revealed positive association between the two miRNAs,with r = 0. 321 between miR-93 and miR-378. Conclusion:miR-93 might serve as a diagnostic molecule with miR-378 in AML.